Despite the increasing importance of nonionic surfactants, methods for their identification have been limited to specific groups. Accordingly, a general analytical scheme for the identification of polyoxyalkylene‐type nonionic surfactants was developed, which uses qualitative tests and two paper chromatographic solvent systems. This procedure differentiates among the ethoxylates of alcohols, thioalcohols, alkyl phenol, fatty acids, polyoxyethylene polyoxypropylene acids, sorbitan fatty esters, fatty amides, polyoxyethylene glycols (PEG) condensates. In addition, individual members of a class can be separated and identified. The method is applicable to a variety of detergent products and to mixtures of more than one nonionic. As little as 7 mg of a nonionic surfactant are sufficient for complete characterization.
159Sunzmary . The intracellular localization of an enzyme system which alters the 17,21d i hydroxy-2 0-ketone side-chain of cortisone was studied in rat liver. The nuclei and mitochondria were inactive. The enzyme was found to be approximately equally distributed between a microsome fraction sedimented at 20,000 x gravity and the supernatant fraction. The enzyme activity was greatly enhanced on addition of magnesium and either glucose-6-phosphate, citrate, or ismitrate. apolis, 1949, p148. 4. Potter, V. R., Recknagel, R. 0.) and Hurlbert, R. B., Fed. Proc., 1951, v10, 646. 5. Tomkins, G., and Isselbacher, K.
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