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Field investigation, Microfacies analysis, and biostratigraphy have been carried out in the central parts of the Ionian Basin (Aetoloakarnania area, Western Greece) in order to decipher the depositional environments that developed during the accumulation of the Upper Cretaceous to Eocene carbonate succession. Three different Standard Microfacies types (SMF) have been observed, corresponding to two different depositional environments (Facies Zones or FZ) of a platform progradation. The three SMF types which occur in the study area during the Upper Cretaceous to Eocene are: 1. SMF 3 that includes mudstone/wackestone with planktic foraminifera and radiolaria, corresponding to toe-of-slope (FZ: 3), 2. SMF 4, which can be classified as polymict clast-supported microbreccia, indicating a toe-of-slope-slope environment (FZ: 4) and 3. SMF 5 which is characterized by allochthonous bioclastic breccia and components deriving from adjacent platforms and which reflects a slope environment. Microfacies analysis provided evidence of a change in the origin of sedimentary components and biota showing the transition from toe-of-slope to slope, as well as a change in organism distribution.