Monoacylglycerol (MAG) and diacylglycerol (DAG) are two natural
components found in most edible oils and fats. Conventional synthesis of
MAG and DAG is usually conducted by glycerolysis of triacylglycerol
(TAG) at high temperatures (above 200 °C) in the presence of an alkaline
catalyst. In this work, the synthesis of MAG and DAG using enzymatic
glycerolysis of olive oil was investigated using Tween 80 as surfactant,
n-butanol as co-surfactant and the novel lipase in free/liquid
formulation Lipozyme TL 100L as catalyst. Experimental design was used
to evaluate the effect of enzyme load and reaction temperature on the
feedstock conversion. Enzyme load and system temperature were
significant variables in the statistical design and the best condition
was found at 35 °C, 7.5 vol% of Lipozyme TL 100 and glycerol to oil
molar ratio of 2:1 with conversion of TAG at approximately 98 % after 2
h of process. A mathematical model based on the Ping-Pong Bi-Bi
mechanism was used to describe the reaction kinetics. The model
adequately described the behavior of the system and can be a useful tool
for the design of reactors in larger scales.
Monoacylglycerol (MAG) and diacylglycerol (DAG) are two natural
components found in most edible oils and fats. Conventional synthesis of
MAG and DAG is usually conducted by glycerolysis of triacylglycerol
(TAG) at high temperatures (above 200 °C) in the presence of an alkaline
catalyst. In this work, the synthesis of MAG and DAG using enzymatic
glycerolysis of olive oil was investigated using Tween 80 as surfactant,
n-butanol as co-surfactant and the novel lipase in free/liquid
formulation Lipozyme TL 100L as catalyst. Experimental design was used
to evaluate the effect of enzyme load and reaction temperature on the
feedstock conversion. Enzyme load and system temperature were
significant variables in the statistical design and the best condition
was found at 35 °C, 7.5 vol% of Lipozyme TL 100 and 2:1 molar ratio
(glycerolysis:oil) with conversion of TAG at approximately 98 % after 2
h of process. A mathematical model based on the Ping-Pong Bi-Bi
mechanism was used to describe the reaction kinetics. The model
adequately described the behavior of the system and can be a useful tool
for the design of reactors in larger scales.
Direitos para esta edição cedidos à Atena Editora pelos autores. Open access publication by Atena Editora Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.