Proof-of-concept is provided that al arge estate of 16-membered macrolide antibiotics can be reached by a"unified" approach. The key building blockwas formed on scale by an asymmetric vinylogous Mukaiyama aldol reaction;i ts alkene terminus was then converted either into the corresponding methyl ketone by Wacker oxidation or into ac hainextended aldehyde by catalyst-controlled branch-selective asymmetric hydroformylation. These transformations ultimately opened access to two structurally distinct series of macrolide targets.N otable late-stage maneuvers comprise ar are example of ar uthenium-catalyzed redox isomerization of an 1,3-enyne-5-ol into a1,3-diene-5-one derivative,aswell as the elaboration of atertiary propargylic alcohol into an acyloin by trans-hydrostannation/Chan-Lam-type coupling.M oreover,t his case study illustrates the underutilized possibility of forging complex macrolactone rings by transesterification under essentially neutral conditions.

Since the accompanying study had shown that the introduction of the eponymous aldgarose sugar to the C5-OH group of the macrocyclic aglycone of aldgamycin Ni sm ost difficult, if not even impossible,the synthesis route was revised and the glycosidation performed at an earlier stage.T omitigate the "cost" of this strategic amendment, apractical and scalable de novo synthesis of this branched octose was developed. The glycoside formation required mild conditions;i tc ommenced with the reaction of the aglycone with the trichloroacetimidate donor to give atransient orthoester,which slowly rearranged to the desired aldgaropyranoside.T he presence of the polar peripheral groups in the product did not impede the selective late-stage functionalization of the macrolide ring itself:t he contained propargylic alcohol entity was readily transformed into the characteristic acyloin motif of the target by ar uthenium-catalyzed trans-hydrostannation followed by amodified Chan-Lam-type coupling.

Microfluidic devices can mimic naturally occurring microenvironments and create microbial population heterogeneities ranging from planktonic cells to biofilm states. The exposure of such populations to spatially organized stress gradients can promote their adaptation into complex phenotypes, which are otherwise difficult to achieve with conventional experimental setups. Here a microfluidic chip that employs precise chemical gradients in consecutive microcompartments to perform microbial adaptive laboratory evolution (ALE), a key tool to study evolution in fundamental and applied contexts is described. In the chip developed here, microbial cells can be exposed to a defined profile of stressors such as antibiotics. By modulating this profile, stress adaptation in the chip through resistance or persistence can be specifically controlled. Importantly, chip‐based ALE leads to the discovery of previously unknown mutations in Escherichia coli that confer resistance to nalidixic acid. The microfluidic device presented here can enhance the occurrence of mutations employing defined micro‐environmental conditions to generate data to better understand the parameters that influence the mechanisms of antibiotic resistance.

The first total synthesis of a tetracyclic marine pyridinium alkaloid hinged on recent advances in chemoselectivity management: While many classical methods failed to afford the perceptively simple pyridine-containing core of the target, nickel/iridium photoredox dual catalysis allowed the critical CÀ C bond to be formed in good yield. Likewise, ring closing alkyne metathesis (RCAM) worked well in the presence of the unhindered pyridine despite the innately Lewis acidic Mo(+ 6) center of the alkylidyne catalyst. Finally, an iridium catalyzed hydrosilylation was uniquely effective in reducing a tertiary amide without compromising an adjacent pyridine and the lateral double bonds; this transformation is largely without precedent. The second strained macrocycle enveloping the core was closed by intramolecular N-alkylation with formation of the pyridinium unit; the reaction proceeded site-and chemoselectively in the presence of an a priori more basic tertiary amine.

The hydantoinase process is applied for the industrial synthesis of optically pure amino acids via whole cell biocatalysis, providing a simple and well-established method to obtain the catalyst. Nevertheless, whole cell approaches also bear disadvantages like intracellular degradation reactions, transport limitations as well as low substrate solubility. In this work the hydantoinase and carbamoylase from Arthrobacter crystallopoietes DSM 20117 were investigated with respect to their applicability in a cell-free hydantoinase process. Both enzymes were heterologously expressed in Escherichia coli BL21DE3. Cultivation and induction of the hydantoinase under oxygen deficiency resulted in markedly higher specific activities and a further increase in expression was achieved by codon-optimization. Further expression conditions of the hydantoinase were tested using the microbioreactor system BioLector®, which showed a positive effect upon the addition of 3% ethanol to the cultivation medium. Additionally, the hydantoinase and carbamoylase were successfully purified by immobilized metal ion affinity using Ni Sepharose beads as well as by functionalized magnetic beads, while the latter method was clearly more effective with respect to recovery and purification factor. Immobilization of both enzymes via functionalized magnetic beads directly from the crude cell extract was successful and resulted in specific activities that turned out to be much higher than those of the purified free enzymes.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0420-3) contains supplementary material, which is available to authorized users.

Since the accompanying study had shown that the introduction of the eponymous aldgarose sugar to the C5-OH group of the macrocyclic aglycone of aldgamycin Ni sm ost difficult, if not even impossible,the synthesis route was revised and the glycosidation performed at an earlier stage.T omitigate the "cost" of this strategic amendment, apractical and scalable de novo synthesis of this branched octose was developed. The glycoside formation required mild conditions;i tc ommenced with the reaction of the aglycone with the trichloroacetimidate donor to give atransient orthoester,which slowly rearranged to the desired aldgaropyranoside.T he presence of the polar peripheral groups in the product did not impede the selective late-stage functionalization of the macrolide ring itself:t he contained propargylic alcohol entity was readily transformed into the characteristic acyloin motif of the target by ar uthenium-catalyzed trans-hydrostannation followed by amodified Chan-Lam-type coupling.

The total synthesis of the 16‐membered macrolide mycinamicin IV is outlined, which complements our previously disclosed, largely catalysis‐based route to the aglycone. This work must also be seen in the context of our recent conquest of aldgamycin N, a related antibiotic featuring a similar core but a distinctly different functionalization pattern. Taken together, these projects prove that the underlying blueprint is integrative and hence qualifies for a collective approach to this prominent class of natural products. In both cases, the final glycosylation phase mandated close attention and was accomplished only after robust de novo syntheses of the (di)deoxy sugars of the desosamine, chalcose, mycinose and aldgarose types had been established. Systematic screening of the glycosidation promoter was also critically important for success.

Proof‐of‐concept is provided that a large estate of 16‐membered macrolide antibiotics can be reached by a “unified” approach. The key building block was formed on scale by an asymmetric vinylogous Mukaiyama aldol reaction; its alkene terminus was then converted either into the corresponding methyl ketone by Wacker oxidation or into a chain‐extended aldehyde by catalyst‐controlled branch‐selective asymmetric hydroformylation. These transformations ultimately opened access to two structurally distinct series of macrolide targets. Notable late‐stage maneuvers comprise a rare example of a ruthenium‐catalyzed redox isomerization of an 1,3‐enyne‐5‐ol into a 1,3‐diene‐5‐one derivative, as well as the elaboration of a tertiary propargylic alcohol into an acyloin by trans‐hydrostannation/Chan‐Lam‐type coupling. Moreover, this case study illustrates the underutilized possibility of forging complex macrolactone rings by transesterification under essentially neutral conditions.