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To control the ignition timing in a gasoline compression ignition (GCI) engine, ozone (O3) was introduced into the intake air. The O-radicals are decomposed from the O3 above 550 K during the compression stroke, and combine into oxygen (O2) in a very short time.The authors adopted two-stage direct injection to mix the fuel injected into the cylinder at very early timings with the O-radicals, before a reduction of the O-radicals would take place. The ignition timing of the second fuel injection for the main combustion is controlled by the heat release from the first fuel injection. In this paper, engine experiments were performed to examine the feasibility of the ignition control with a primary reference fuel, octane number 90 (PRF90). The O3 concentration, the quantity, and the timing of the first injection were changed as experimental parameters. The results showed that a very small quantity of O3, tens of ppm, is sufficient to promote the heat release of the first injected fuel. The heat release increases with the O3 concentration and