The results suggested that local application of the mucoadhesive multiple layer films loaded with metformin hydrochloride was able to manage moderate chronic periodontitis.

Drynaria fortunei demonstrated increased regeneration and bone formation when used in the treatment of furcation defects in a canine model.

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INTRODUCTION:The use of platelet concentrates remains controversial. Several techniques for platelet concentrates are available and each method leads to a different product with different biology and potential uses. Platelet rich fibrin (PRF) is an autologous biomaterial composed of a fibrin clot matrix entrapping leukocytes, cytokines, living progenitor cells, and platelets capable of releasing various growth factors. One of the recent platelet concentrates is the mineralized plasmatic matrix (MPM) which has a different way of preparation. It creates a stable homogeneous single-moldable compound. OBJECTIVES: The aim of this experimental study was to compare the effect of PRF to MPM when combined with biphasic calcium phosphate alloplast in treating horizontal critical sized ridge defects in a canine model. MATERIALS AND METHODS: A split mouth design was carried out using the third and fourth mandibular premolars of two healthy mongrel dogs. A total of eight horizontal critical sized ridge defects were surgically created. The defects on the left side (group I); were managed with MPM composite, MPM compact layer, then collagen membrane. On the right side (group II); defects were managed with PRF mixed with biphasic calcium phosphate alloplast followed by the application of PRF compact strips, then collagen membrane. The dogs were sacrificed after 4 weeks. Samples were dissected and prepared for histological evaluation. RESULTS: Histological results showed that quality of bone formed in MPM group was superior to PRF. This was evident with the formation of well-organized compact bone in MPM specimens versus immature woven trabecular bone in PRF ones. CONCLUSIONS:The fibrin network that is produced in the MPM composite allows for its homogeneity. This offers a very good stability for the graft which was found to be more effective in enhancing wound healing during guided bone regeneration of horizontal ridge defects.

BackgroundPeriodontal regenerative therapy using bone‐substituting materials has gained favorable clinical significance in enhancing osseous regeneration. These materials should be biocompatible, osteogenic, malleable, and biodegradable. This study assessed the periodontal regenerative capacity of a novel biodegradable bioactive hydrogel template of organic–inorganic composite loaded with melatonin.Materials and methodsA melatonin‐loaded alginate‐chitosan/beta‐tricalcium phosphate composite hydrogel was successfully prepared and characterized. Thirty‐six critical‐sized bilateral class II furcation defects were created in six Mongrel dogs, and were randomly divided and allocated to three cohorts; sham, unloaded composite, and melatonin‐loaded. Periodontal regenerative capacity was evaluated via histologic and histomorphometric analysis.ResultsMelatonin‐treated group showed accelerated bone formation and advanced maturity, with a significant twofold increase in newly formed inter‐radicular bone compared with the unloaded composite. The short‐term regenerative efficacy was evident 4 weeks postoperatively as a significant increase in cementum length concurrent with reduction of entrapped epithelium. After 8 weeks, the scaffold produced a quality of newly synthesized bone similar to normal compact bone, with potent periodontal ligament attachment.ConclusionsMelatonin‐loaded hydrogel template accelerated formation and enhanced quality of newly formed bone, allowing complete periodontal regeneration. Furthermore, the scaffold prevented overgrowth and entrapment of epithelial cells in furcation defects.