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BACKGROUND Enterobacteriaceae is the primary pathogen isolated predominantly among gram negatives. The prevalence of multidrug-resistant Enterobacteriaceae has increased continuously over the past few years. Extended-spectrum β-lactamases (ESBLs) are of particular concern as the rate of resistance has increased over the years. We wanted to characterise Enterobacteriaceae and antibiotic sensitivity patterns with special reference to detection of ESBL. MATERIALS AND METHODS The present study was conducted in the microbiology laboratory of Sree Gokulam Medical College and Research Foundation over a duration of 3 months from Oct 2015 to Dec 2015. Using standard techniques and CLSI protocol we identified the organisms with antibiotic sensitivity. RESULTS 20% of Enterobacteriaceae were ESBL positive with E coli being the predominant organism. CONCLUSION 20% ESBL production was less compared to studies from other parts of the country.