Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, alternative material is required in order to cope with this bacterium, which was by using natural material such as kersen leaf (Muntingia calabura L). The purpose of the research is to determine the effect of secondary metabolite contained in kersen leaf as anti-bacterial against Aeromonas hydrophila. This research used a method of MIC (Minimum Inhibitory Concentration) as an anti-bacterial test. In order to examine the secondary metabolite content, phytochemical screening and FTIR (Fourier Transform Infrared Spectroscopy) methods were used. The results obtained through the MIC test of 125 ppm is the minimum concentration capable of inhibiting the growth of Aeromonas hydrophila. Kersen leaf extracts is bacteriostatic antibacteria which only inhibit bacterial growth up to 24 hours. Phytochemical screening reported that kersen leaf extracts were positive of flavonoids, tannins, saponins, alkaloids and triterpenoids. Through FTIR test, it is known that phenol compounds were contained in kersen leaf extracts. This phenol is one of the metabolites with the function of anti-bacterial on this study.
This research aims to investigate the influence of Ketapang (Terminalia catappa) bark extract (KBE) on the survival rate and liver histopathology of Common carp (Cyprinus carpio) which was Infected by Aeromonas hydrophilia. Experimental design with Completely Randomized Design (CRD). Obtained data were analyzed by using Analysis of Varian (ANOVA) and further Least Significant Differed (LSD) analysis. The main parameters in this research are the damage level of Common carp liver based on scoring value and survival rates. Based on the result from scoring data, it was shown that there is altered-histology on Common carp fish liver. The damages found were congestion, melanomacrofag, and necrosis. In average, treatment A (730 ppm) had the highest damage average while treatment C (770 ppm) had the lowest damage average value. The final result shows that the bigger the dose, the lower the damage. In contrast, the survival rates would decrease with the higher dose of KBE. It can be concluded that KBE able to reduce the liver alteration however in some extend it will cause the mortality.Keywords : common carp, histopathology, ketapang, liver, survival rate.
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