Many infectious diseases in ancient times have been treated using herbal drugs. Today efforts are made for the discovery of the herbal components from plants possessing antimicrobial properties. Herbal drugs are widely used for treatment of various diseases, and they play an important role in health care considerations. Rhizome of Inchnocarpus frustescens were extracted, phytochemically investigated and were subjected to antibacterial as well as antifungal screening by Disk Diffusion Method. The Minimum Inhibitory Concentration (MIC) was also performed by two-fold tube dilution method. Three compounds were isolated and identified as n-nonadecanyl benzoate (1), benzocosanyl arachidate (2), and Quercetin (3). The result of phytochemical investigation showed that compound (3) is new isolated the first time from rhizomes; and compounds (1)and (2) are isolated from genus Ichnocarpus frutescens form other morphological parts earlier. Further antimicrobial tests indicated that the ethanolic extract inhibited the growth of all microorganisms and most extracts showed promising degree of antimicrobial activity. The results were compared with standard antimicrobial agents (Erythromycin and Fluconazole), Thus providing evidence for the potential use of these crude extract in the treatment of bacterial and fungal infections.
Objective: The study deals with the antimicrobial activity of five solvent extracts of roots of Caesaria tomentosa against seven microorganisms: two gram-positive (Staphylococcus aureus and Bacillus subtilis), three gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimrium) and two fungal strains (Candida albicans and Aspergillus niger). Methods: Roots of C. tomentosa were extracted with different solvents(n-hexane, ethanol, chloroform, acetone and water) and were subjected to antibacterial as well as antifungal screening by Well Diffusion Method. The Minimum Inhibitory Concentration(MIC) was also performed by two-fold dilution. Results: The maximum inhibition zone at 50μg/ml concentration of n-hexane was 32 mm. These indicate that some active substances in C. tomentosa dissolved in varying degrees in the five solvents. The MIC for n-hexane was 1.6 μg/ml for S. aureus leading to a conclusion that the n-hexane extract was found to be the most potent. Conclusion: All extracts were very effective against S. aureus. As for the solvents, the n-hexane extract had the best inhibitory effect among five solvents tested.
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