One-half million cases of septicemia are reported annually with a mortality of around 35%.Diagnosis depends in part on blood cultures which require one to two weeks.It would be advantageous if an early sign of septicemia were av a. i 1a.b1 e.Cytoplasmic vacuolation of polymorphonuclear neutropnils is occasionally seen in the peripheral blood smears of 2 patients who have infections. The object of this work was to determine the cause and si9nif icance of cytoplasmic vacuolation with the 9oal of usin9 the occurrence of vacuolation as an early indicator of infection.It was necessary first to establish that the phenomenon was not simply an artifact of slide preparation techni- ---------------- Spontaneous formation of cytoplasmic vacuoles in neutrophi ls in wet mounts was reported by Gordin <1952> and by Bessis <1972>. Gordin <1952> noted that vacuole size increased at a constant rate, once formation had begun, leading him to the conclusion that vacuole formation was due to a physical process not related to cellular metabolism. Solberg and Hellum <1972> examined neutrophils from bacterially infected patients and found that they were able to ingest but were less able to kill bacteria in vivo than were neutrophils taken from afebrile patients. induces artifacts, includin9 vacuolation, which render blood smears useless for dia9nostic purposes. However, several hospitals in this metropolitan area make their dia9nosticsmears from anticoa9ulated blood, usually usin9 EDTA, as anticoa9ulant.Since, in some phases of my work it was easiest to obtain anticoa9ulated blood and since it seemed stran9e that technolo9ists who warned of anticoa9ulant artifact induction were, in fact, usin9 anticoa9ulated blood for their smears, this study was undertaken to ascertain the vacuole-inducin9 capability of various anticoa9ulants 9iven prolon9ed ~n ~~i~g exposure.
MATERIALS AND METHODSTen 10 ml tubes of blood were drawn from a normal healthy subject. Pairs of tubes contained the followin9 anticoa9ulants: sodium heparin, sodium oxalate, potassium oxalate/ sodium fluoride, EDTA, and sodium citrate. As a control, needle-tip smears were made usin9 no anticoa9ulant. Table I. were scored for maturity and presence of vacuoles. The fifty-nine hospital inpatients who were selected for presence of vacuolation had a mean percent vacuolation of 17.6% scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health. hi@scite.ai 10624 S. Eastern Ave., Ste. A-614 Henderson, NV 89052, USA Copyright © 2024 scite LLC. All rights reserved. Made with 💙 for researchers Part of the Research Solutions Family.. Nine <19%) of the patients with vacuolation
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