Rhodamine‐based reversible fluorescence turn‐on chemosensor appended with ‘N’ and ‘O’ donor atoms as chelating moieties has been synthesized for the selective detection of Fe3+ ion over other competitive metal ions in aqueous medium. Designed probe indicated color change to pink with lower limit of detection at 1.93×10−7 M exhibiting high sensitivity toward Fe3+ ion. Sensing mechanism was established by HRMS and NMR titration studies. The probe was successfully applied for the detection of Fe3+ ions in live MCF‐7 cells.
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