Psoriasis is a chronic skin disease characterized by hyperproliferation and impaired differentiation of epidermal keratinocytes accompanied by increased inflammation, suggesting that molecules with antiproliferation and anti-inflammatory abilities may be effective for its treatment. One of the key steps in regulating cell proliferation is DNA replication initiation, which relies on prereplication complex (pre-RC) assembly on chromatin. CDC6 is an essential regulator of pre-RC assembly and DNA replication in eukaryotic cells, but its role in proliferation of keratinocytes and psoriasis is unknown. Here we examined CDC6 expression in psoriatic skin and evaluated its function in the proliferation of human keratinocytes. CDC6 expression is upregulated in epidermal cells in psoriatic lesions and it could be induced by IL-22/STAT3 signaling, a key signaling pathway involved in the pathogenesis of psoriasis, in keratinocytes. Depletion of CDC6 leads to decreased proliferation of keratinocytes. We also revealed that berberine (BBR) could inhibit CDK4/6-RB-CDC6 signaling in keratinocytes, leading to reduced proliferation of keratinocytes. The mechanism of antiproliferation effects of BBR is through the repression of JAK1, JAK2, and TYK2, which in turn inhibits activation of STAT3. Finally, we demonstrated that BBR could inhibit imiquimod-induced psoriasis-like skin lesions and upregulation of CDC6 and p-STAT3 in mice. Collectively, our findings indicate that BBR inhibits CDC6 expression and proliferation in human keratinocytes by interfering the JAK–STAT3 signaling pathway. Thus, BBR may serve as a potential therapeutic option for patients with psoriasis.

The grounding grid plays an important role in the safe and reliable running of substation, The study on detection and fault diagnosis of grounding grid will provide an effective means for its state evaluation. This paper proposes a multiple parameter imaging method for grounding grid by pulsed electromagnetic inverse, that aims at accurate positioning and quantitative evaluation of corrosion and breakpoint for power system grounding grid, and the new method intends to realize the image reconstruction of electrical conductivity, permittivity and structure for grounding grid. Using the characteristics of electromagnetic wave propagating in different medium surface, the new method establishes the mathematical-physical model and analyzes the electromagnetic field propagation characteristics between grounding grid and the surrounding medium.

Gas insulated switchgear (GIS) is an important electric power equipment in a substation, and its running state has a significant relationship with stability, security, and reliability of the whole electric power system. Detecting and analyzing the decomposition byproducts of sulfur hexafluoride gas (SF6) is an effective method for GIS state assessment and fault diagnosis. This paper proposes a novel gas sensor based on flower-like ZnO nanorods to detect typical SF6decompositions. Flower-like ZnO nanoparticles were synthesized via a simple hydrothermal method and characterized by X-ray powder diffraction and field-emission scanning electron microscopy, respectively. The gas sensor was fabricated with a planar-type structure and applied to detect SF6decomposition products. It shows excellent sensing properties to SO2, SOF2, and SO2F2with rapid response and recovery time and long-term stability and repeatability. Moreover, the sensor shows a remarkable discrimination among SO2, SOF2, and SO2F2with high linearity, which makes the prepared sensor a good candidate and a wide application prospect detecting SF6decomposition products in the future.

In this study, the influence of incremental rate of anodising current V IR on the crystal structure, surface roughness and corrosion resistance of anodic oxide films on Ti-10V-2Fe-3Al was studied using Raman spectroscopy, atomic force microscopy (AFM), electrochemical impedance spectroscopy (EIS) and potentiodynamic polarisation. The results showed that the surface roughness, crystal structure and electrochemical corrosion behaviour of anodic oxide films were remarkably affected by different V IR with regard to the change in the rate of anodising voltage. In addition, results of EIS and potentiodynamic polarisation revealed that the film that formed at V IR of 0?50 A min 21 had the highest corrosion resistance. Quantitative AFM characterisation showed good correlation between surface roughness and EIS results. Furthermore, results of Raman spectroscopy showed that the relative intensity of anatase and rutile had a tendency to increase with a decrease in V IR before reaching 0?50 A min 21 .

No abstract