An aqueous nanoparticle suspension of a bis-methanophosphonate fullerene (n-BMPF) was tested to clarify its effects on polymerase chain reaction (PCR) with the catalyst of Taq DNA polymerase and DNA exonuclease Exo Ⅲ and the template of super-coiled plasmid pEGFP-N1. It was found that the product amounts from PCR decreased significantly with addition of the n-BMPF. The inhibition by the n-BMPF was dose-dependent and IC50 values for reactions of PCR were 2.7 μmol/L. Increase of Taq DNA polymerase amounts in PCR system antagonized the activities of the n-BMPF. However, addition of two scavengers of reactive oxygen species (ROS), mannitol and azide at the concentrations of 2~10 mmol/L did not antagonize the activities of the n-BMPF against PCR. These data implied that this inhibition probably did not correlate to ROS. Meanwhile, the inhibition for the DNA exonuclease Exo Ⅲ by the n-BMPF was evident and dose-dependent.
A 3-state Markov model is used to model some safety-critical systems, when the system states can be divided into three kinds of categories: Operating States, Failed-Safe States & Failed-Unsafe States. These models are used to estimate the system safety and security. Based on these models, a new model is built when the system fails, different defect states have different measures. For Failed-Safe States, the repair process is adopted and for the other failed states is not. The repeatedly repairs have some influences on the operating times, repair times and the coverage to different failed states. Geometric Process is employed to describe this repair process and get some indexes of the system safety.Operational State at time t Probability that the system is in the Failed-Safe State at time t Probability that the system is in the Failed-Unsafe State Probability vector for the Markov model at time t Transition rate matrix of the Markov process Safety function at time J:
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.