The present work describes the application of new spectrophotometric methods to the determination of osmium in the intermetallides Nd 20 Os 15 Sі 65 and Nd 10 Os 30 Sі 60. The methods are based on the interaction of osmium with azo dyes (Tropaeolin O, Tropaeolin OOO-I, Eriochrome Black T), flavonoids (morin and quercetin) or Tiron. They are simple and rapid. The sensitivity varies from 0.005 tо 0.8 µg ml-1 ; the effective molar absorptivity is ~10 3-10 5 M-1 ⋅ ⋅ ⋅ ⋅сm-1 , depending on the reagent. Intermetallides / Osmium(IV) / Spectrophotometric determination / Organic reagents
Osmium assay in fixatives and stained rat tissues by means of acid and o,o′-dihydroxo substituted monoazo dyes and some flavonoids
Research ArticleAbstract: New simple, rapid and accurate spectrophotometric techniques for osmium assays in fixatives and stained tissues have been elaborated using acid (Tropaeolin O, Tropaeolin OOO-I, Tropaeolin OOO-II, Orange G), o,o′-dihydroxo substituted azo dyes (Eriochrome Blue Black R, Acid Chrome Dark-Blue, Eriochrome Black T) and flavonoids (morin, quercetin, luteolin). Methods are based on sensitive osmium(IV) reactions with reagents and the formation of stable coloured compounds. Tolerance ratios of main matrix components of histological specimens during Os(IV) spectrophotometric determination with reagents has been established. Results of osmium determination in fixatives and fixed tissues, obtained by means of different reagents, appeared to be sufficiently similar, although effects of matrix components differ. The accuracy of spectrophotometric osmium assay in fixatives with azo dyes has been confirmed with voltammetric investigations. Results obtained from osmium uptake by rat gum tissues are valuable for clinical testing of dental drugs, indicating the influence of drug treatment on the gums.
The interaction of Os(IV) ions with morin, quercetin, luteolin has been investigated and the spectrophotometric methods of osmium determination using these reagents have been elaborated. The influence of various factors and the maximum yield of the products of Os(IV) ions interaction with flavonoids have been determined. The change of electronic absorption spectra depending on the duration of the solution heating in a boiling water bath in the presence or absence of atmospheric oxygen as well as the interaction of osmium(ІІ, ІІІ, VІ and VІІІ) ions with flavonoids investigated have been shown. The redox nature of osmium(IV) ions interaction with morin and quercetin has been confirmed.
The paper describes the preparation of new analytical forms of rhodium(III) compounds with flavonoids (morin and quercetin) and its rapid spectrophotometric determination in the intermetallic compounds HoRhIn 5 and LaRhIn 2. The concentration ranges of the rhodium ions with morin and quercetin were equal to 0.08-1.50 µg/ml (С min = 0.030 µg/ml) and 0.05-2.55 µg/ml (С min = 0.012 µg/ml), respectively.
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