In this work, novel magnetic molecularly imprinted polymers were prepared for the selective extraction of osthole from Libanotis Buchtomensis herbal extract. During the synthesis process, double bonds grafted on the surface of Fe3 O4 nanoparticles could not only drive the temple molecules to locate onto the surface of vinyl-functionalized magnetic nanoparticles by π-π conjugation, which makes the distribution of binding sites ordered, but also direct the occurrence of imprinting polymerization at the surface of magnetic nanoparticles by the copolymerization of vinyl terminal groups with functional monomers and cross-linking agent. The characteristics of the resulting polymers were evaluated by transmission electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and vibrating sample magnetometry. Adsorption kinetics, isotherms, selectivity, reproducibility, and reusability were discussed, which suggest that the obtained nanomaterials possess rapid binding kinetics, high adsorption capacity of 17.65 mg/g, and favorable selectivity for the target molecule. Satisfactory reproducibility and reusability were verified as well. Meanwhile, the resultant imprinted nanoparticles were successfully applied to selectively separate osthole from the herbal extract, which show great potential in extracting active ingredients from traditional Chinese medicine.
We report a core-shell magnetic molecularly imprinted polymer with high affinity through a facile sol-gel method for the selective adsorption of bovine hemoglobin from real bovine blood. Copper ions grafted on the surface of the matrix could immobilize template protein through chelation, which greatly enhances the orderliness of imprinted cavities and affinity of polymers. The obtained products exhibit a desired level of magnetic susceptibility, resulting in the highly efficient adsorption process. The results of adsorption experiments show that the saturation adsorption capacity of imprinted products could reach 116.3 mg/g within 30 min. Meanwhile, the specific binding experiment demonstrates the high selectivity of polymers for bovine hemoglobin. Furthermore, satisfactory reusability is demonstrated by ten adsorption-desorption cycles with no obvious deterioration in binding capacity. Electrophoretic analysis suggests the polymer could be used successfully in separation and enrichment of bovine hemoglobin from the bovine blood sample, which exhibits potential application in pretreatment of proteomics.
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