Abstract.-The loss of protein synthesis during early mouse-brain development was shown to be the result, at least in part, of the inability of microsomes obtained from more mature neural tissue to participate in rapid polypeptide synthesis. The loss of brain microsomal activity was observed shortly after birth and continued until the animals were approximately ten days old. Despite the difference in synthetic activity, sucrose gradient profiles of microsomes and polyribosomes from young and more mature brain tissue were quite similar. The loss in protein synthesis was shown to be independent of available mRNA and not attributable to aminoacyl-RNA synthetases and tRNA binding activity.Introduction.-Several studies have indicated that the rates of macromolecular synthesis are altered during maturation of mammalian brain tissue. Higher rates of protein synthesis in younger brain tissue have been observed both in vivo and in vitro. 16 We have reported that both protein and RNA synthesis undergo a rapid decrement during early development of mouse brain tissue. The loss of protein synthesis during this critical period of development has been shown to be the results of a change in an intracellular component rather than an alteration in cellular membrane permeability.7 The present report involves an investigation of the identity of the intracellular component that is responsible, at least in part, for this altered rate of macromolecular synthesis. The role of microsomes and polyribosomes in this developmental phenomenon is described and compared to similar studies with brain and other mammalian tissues.
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