Background: Neonatal sepsis is a prime cause of neonatal morbidity and mortality. Peripheral venous blood (PVB) culture is a gold standard for the diagnosis of neonatal sepsis. Low sensitivity of blood culture in newborn is due to small volume of blood sample and antibiotics given before sampling.
Aims and Objectives: The study aims to compare and analyze the microbial spectrum in the Umbilical cord blood (UCB) culture with PVB culture and evaluation of both for detection of early onset neonatal sepsis (EONS) in high risk neonates and to use appropriate microorganism specific antibiotic for treating the sepsis.
Materials and Methods: About 100 inborn neonates with two or more risk factors for EONS, chosen by sequential sampling method were included in this prospective analytical study. Blood samples were collected from umbilical cord and peripheral vein for culture. Sepsis screen was done to substantiate the diagnosis of neonatal sepsis.
Results: Out of 100 neonates, 21 belongs to sepsis; 14 to probable sepsis; 65 to no sepsis. Among these 27 had UCB culture & 24 had PVB culture +ve. Organisms grown on culture were Klebsiella(m/c isolate), Staphylococcus aureus, Escherichia coli, Acinetobacter, Coagulase-ve Staphylococcus (blood culture contaminant), Pseudomonas and Enterococcus.
Conclusion: UCB culture is simple and convenient method for the diagnosis of neonatal sepsis as compared to PVB culture with Sensitivity of 66% and specificity of 93%. Organisms grown are comparable to PVB culture sample (Klebsiella - most common isolate).
Background: Sepsis is one of the major causes of neonatal morbidity and mortality. Early recognition and diagnosis of early-onset neonatal sepsis (EONS) is difficult because of the variable and non-specific clinical presentation of this condition. Hence, there is a need for early predictive screening method for EONS.
Aims and Objectives: To compare the umbilical cord blood Haematological Scoring System (HSS) with peripheral venous blood as an early predictive screening method for detection of EONS.
Materials and Methods: 100 inborn neonates with two or more risk factors for EONS, chosen by sequential sampling method were included in this prospective analytical study. Blood samples were collected from the umbilical cord and peripheral vein analyzed for hematological parameters, sepsis screen, and peripheral smear for HSS of Rodwell et al., send for blood culture. Blood cultures were performed as gold standard for diagnosing neonatal sepsis and sepsis screen was done to corroborate the diagnosis of neonatal sepsis.
Results: Of 100 neonates, 21 belongs to sepsis; 14 to probable sepsis; 65 to no sepsis. HSS in umbilical cord blood (UCB) had Sensitivity-74.28%, Specificity-92.30%, PPV-83.87%, NPV-86.95% and HSS in PVB had Sensitivity-62.85%, Specificity- 87.69%, PPV-75.86%, NPV-81.69%.
Conclusion: HSS score in UCB can be used as a simple, quick, cost-effective, and readily available screening test with decent sensitivity and high specificity, for the detection of EONS.
BACKGROUND : Neonatal sepsis is one of the major causes of neonatal morbidity and mortality.
Peripheral venous blood culture and sensitivity is gold standard for the diagnosis of neonatal sepsis. Low
sensitivity of blood culture in newborn is due to small volume of blood sample collected from neonates & antibiotics given
before sampling.
AIM : To evaluate the utility & compare the Umblical cord blood culture(UCBC) with Peripheral venous blood culture(PVBC) for
detection of Early Onset Neonatal Sepsis(EONS)
METHOD : 100 inborn neonates with two or more risk factors for EONS, chosen by sequential sampling method were included in
this prospective analytical study. Blood samples were collected from umbilical cord and peripheral vein for culture. Sepsis
screen was done to corroborate the diagnosis of neonatal sepsis.
RESULT : Out of 100 neonates, 21 belongs to sepsis; 14 to probable sepsis; 65 to no sepsis. UCBC had Sensitivity-65.71% ,
Specicity-93.84%, PPV-85.18% , NPV-83.56% & PVBC had Sensitivity-60% , Specicity-95.38% , PPV-87.5% , NPV-81.57%.
CONCLUSION : UCBC is simple and convenient method for the diagnosis of EONS compared to PVBC. Organisms grown are
comparable to PVBC sample.
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