Mechanically activated Piezo2 channels are key mediators of light touch and proprioception in mice and humans. Relatively little is known about what other proteins regulate Piezo2 activity in a cellular context. TACAN (TMEM120A) was proposed to act as a high threshold mechanically activated ion channel in nociceptive dorsal root ganglion (DRG) neurons. Here we find that TACAN co-expression robustly reduced mechanically activated Piezo2 currents, but did not inhibit mechanically activated Piezo1 and TREK1 currents. TACAN co-expression did not affect cell surface expression of either Piezo1 or Piezo2 and did not have major effects on the cortical actin or tubulin cytoskeleton. TACAN expression alone did not result in the appearance of mechanically activated currents above background. In addition, TACAN and Piezo2 expression in DRG neurons overlapped, and siRNA mediated knockdown of TACAN did not decrease the proportion of slowly adapting mechanically activated currents, but resulted in an increased proportion of rapidly adapting currents. Our data do not support TACAN being a mechanically activated ion channel, and identify it as a negative modulator of Piezo2 channel activity.

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Webinars are seminar activities conducted online, using certain internet-based websites or applications. Interest is defined of liking or pleasure and being interested in an object or activity without orders from other people. Mahadana Asta Futures is including the oldest brokerage firms in Indonesia that has been certified by CoFTRA. In this era of the covid-19 pandemic, many people want to know about trading because during this pandemic, many people have their economy hampered. With that, Mahadana held a webinar through the Zoom application to meet the public's need for trading. This study uses a quantitative research approach with survey research methods using questionnaires as a method of collecting data distributed to respondents who are participants of the Zoom webinar. Based on resarchist research, it’s found that there is a big significant effect of the existence of the webinar on public interest in joining the international trading business. Webinar adalah seminar yang biasanya dilakukan oleh sebagian orang menggunakan sebuah aplikasi yang rata-rata orang di Indonesia menggunakan aplikasi Zoom untuk medianya. Minat diartikan suka atau senang dan tertarik terhadap sebuah objek atau aktivitas tanpa perintah dari orang lain. Mahadana Asta Berjangka termasuk perusahaan pialang tertua di Indonesia yang telah mendapatkan legalitas oleh Bappebti. Pada era pandemi ini, tentunya para masyarakat yang ingin mengetahui tentang trading dikarenakan terhambatnya perekonomiannya masyarakat. Dengan itu pihak Mahadana menyelenggarakan webinar melalui aplikasi Zoom untuk memenuhi kebutuhan masyarakat akan trading. Penelitian ini peneliti teliti dengan pendekatan penelitian kuantitatif menggunakan metode survei menggunakan kuisioner sebagai salah satu cara untuk mengumpulkan data yang peneliti sebar kepada responden yang menjadi peserta Zoom webinar. Berdasarkan penelitian ini, didapatkan hasil bahwa terdapat pengaruh signifikan dari webinar terhadap minat masyarakat untuk bergabung kedalam bisnis trading internasional yang didapat dari hasil uji koefisien korelasi sebesar 0,725.