Calcium, boron and potassium are important nutrients in plant growth and development, contributing to cell division, cell wall lining, nutrient transport via the plasma membrane, enzymatic activation, nutrient and metabolic translocation via xylem and phloem, biosynthesis and metabolism of carbohydrates, pigments, chromoproteins among other effects. The objective of this work was to demonstrate the physiological effects of Ca, B, and K on the initial growth of Mentha spicata (L.). For the development of this work, a research and bibliographic review was carried out with preliminary tests carried out in 2017/2018 in the Multidisciplinary laboratories of Chemistry, Biochemistry in the premises of the Unigran Capital Faculty, Campo Grande - MS. Mentha spicata (L.) was used to investigate the effects of Ca, B and K, for seedling height, seedling stem diameter, plant tissue analysis, leaf area and chlorophyll, number of stomata and seedling fresh weight. Among the results obtained, it is highlighted that the nutrient solution at concentrations of 0.75% and 1% contributed to the increase in seedlings, average length of roots, shoots, leaf area and fresh mass. Verifying the essential oil yield in the leaves and stems there were no significant differences in the treatments.
O cálcio, boro e potássio são nutrientes importantes no crescimento e desenvolvimento das plantas, contribuindo na divisão celular, revestimento da parede celular, transportes de nutrientes via membrana plasmática, ativação enzimática, translocação de nutrientes e metabólicos via xilema e floema, biossíntese e metabolismo de carboidratos, pigmentos, cromoproteínas dentre outros efeitos. O objetivo deste trabalho foi demonstrar os efeitos fisiológicos do Ca, B, e K no crescimento inicial de Menta spicata (L.). Para o desenvolvimento deste trabalho foi realizada pesquisa e revisão bibliográfica com ensaios preliminares executados em 2017/2018 nos laboratórios Multidisciplinares de Química e Bioquímica nas dependências da Faculdade Unigran Capital, Campo Grande/MS. Utilizou-se Menta spicata L. na investigação dos efeitos do Ca, B e K, para a altura das plântulas, diâmetro caulinares das plântulas, análise dos tecidos vegetais, área foliar e clorofila, número de estômatos e peso fresco das plântulas. Dentre os resultados obtidos destaca-se que a solução nutritiva nas concentrações de 0,75% e 1% contribuíram para o aumento das plântulas, comprimento médio das raízes, brotações, área foliar e massa fresca. Verificada o rendimento do óleo essencial nas folhas e caules não tiveram diferenças significativa nos tratamentos.
Introduction: Meningococcal disease (MD) is caused by the bacterium Neisseria meningitidis, an exclusively human pathogen classified into different serogroups, with A, B, C, Y, W and X being the most associated with epidemic outbreaks around the world. Features such as a rapid progression of the disease combined with a high lethality rate, ranging from 7% to 70% of untreated cases, associated with physical or neurological sequelae after treatment, in up to 20% of cases, demonstrates the importance of the surveillance, prophylaxis and treatment methods for this disease. It is estimated that in Brazil, an endemic region, MD is responsible for a lethality of 21.9%. Currently there are vaccines against different serogroups of Neisseria meningitidis, however, studies indicate that a vaccine developed from de-Oacetylated (oatC-) strains against serogroup C confer IgG levels twice higher and a bactericidal effect more pronounced than vaccines produced from O-acetylated lineages (oatC+).Objective: To evaluate the evolution of the oatC gene responsible for the acetylation of the capsular polysaccharide of Neisseria meningitidis serogroup C of Brazilian isolates from 1991 to 2019, to determine the proportion of oatC+ and oatC-circulating strains in the country.Methodology: With this purpose, a selection of the strains deposited in the Collection of Reference Bacteria in Sanitary Surveillance (CBRVS) was performed. These strains were characterized by molecular methods and sequencing of the oatC gene for classification into oatC+ or oatC-by comparison of deduced amino acid sequences with reference strains for each capsular type. Results:The study showed that the proportion of oatC-circulating strains was 23%, well above the average of 13.5% found in the United Kingdom and United States, the only countries where this type of study has ever been conducted. Conclusion:It was concluded that the introduction of a conjugated serogroup C vaccine produced from oatC-strains could bring benefits to the sensitive population.
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