Cisplatin treatment caused a significant increase in the life span of ascites Dalton's lymphoma (DL) Tumor-bearing (TB) mice. However, as compared to cisplatin (CP) alone, combination treatment with ascorbic acid plus CP resulted in better therapeutic efficacy against murine DL. Cisplatin treatment of TB mice resulted in the appearance of thickened and irregular arrangement of mitochondrial cristae in the liver, kidney and DL tumor cells. Combination treatment of the hosts with ascorbic acid and CP lessened deformities in the mitochondria of liver and kidney, while in tumor cells, this increased the formation of vacuoles and disruption in mitochondrial cristae. Cisplatin treatment decreased the succinate dehydrogenase (SDH) activity in the mitochondria of kidney and DL cells and combination treatment caused further decrease in SDH activity in kidney and DL cells during 24-48 h of treatment. After CP treatment, the protein content in the mitochondria of these tissues decreased, and during combination treatment, it showed significant improvement. Mitochondrial lipid peroxidation (LPO) increased in these tissues after CP treatment. However, combination treatment significantly decreased mitochondrial LPO in liver and kidney but increased in DL cells. This increase in mitochondrial LPO in DL cells and decrease in liver and kidney could play an important role in the antitumor activity of combination treatment and at the same time reduce CP-induced toxicity in the host. However, further study may be desirable to explore some aspects of the mechanism(s) involved in these changes in mitochondria.
Ginger soft rot disease caused by fungal pathogens have become one of the most serious problems causing reduced production around the world. It has also caused a major problem among farmers of Mizoram state in India resulting in a huge decline in rhizome yield. However, the exact causative agents of this disease have not been identified in the state. Therefore, the present study was undertaken to isolate and characterized the causative agents of ginger soft rot disease from the diseased plants collected from five different villages of Aizawl district, Mizoram. Isolated fungi were cultured and morphological and molecular identification were done using internal transcribed spacer of rDNA. Fusarium oxysporum, F. solani and Plectosphaerella cucumerina were identified in ginger samples from five villages. Fusarium spp. were the most common and seem to be the major causative agents. It is suggested that further investigation is required to explore the diversity of ginger soft rot pathogenic fungi in the whole state which could be helpful in introducing effective and eco-friendly disease management programme.
Ginger (Zingiber officinale Rosc.,Zingiberaceae) is an important crop grown in India known for its therapeutic uses. Wilting caused by bacteria, soft rot and yellow disease caused by fungi are the major diseases affecting Ginger production and its cultivation hampering its growth and development. The current study emphasize on the morphological and molecular identification of fungal pathogens causing soft rot disease in ginger that have become one of the major problem among farmers of Mizoram, India as a result of which resulted in a huge decline in rhizome yield and its production. Nevertheless, due to limited studies of the medicinal plants the causative agents associated with these plants are not available. The objective of this study is to investigate the diversity of fungi that cause soft rot in ginger. Twenty fungi were successfully isolated from four villages of Champha iDistrict, Mizoram. The fungi isolated from the infected rhizome were cultured and identification was carried out using morphological characteristics and molecular analysis of DNA sequence generated from Internal Transcribed Spacer rDNA region. Of the samples collected, F. solani,F. oxysporum, P. myriotylum were the most common causative agents. Much research work has yet to be undertaken explore the diversity of pathogrnic fungi causing soft rot in Mizoram which could be very significant in introducing competent and eco-friendly disease management programme.
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