Objective
The use of agarose in nucleic acid electrophoresis is the gold standard. However, agarose is very expensive and not readily available in resource limited developing countries like Ghana. Hence, finding a more affordable and readily available alternative to agarose will be a major boost to molecular research in developing countries. This study was aimed at investigating the use of corn starch as a potential substitute for agarose in DNA gel electrophoresis.
Results
Genomic deoxyribonucleic acid (DNA) extracted from Plasmodium falciparum and primers were obtained from the West African Centre for Cell Biology of Infectious Pathogens and amplified using polymerase chain reaction. The amplicon was run on agarose gel to ascertain the molecular weight (as a positive control). When visualized under both blue light and ultraviolet light, the DNA and ladder showed clear and clean bands with the expected molecular weight. Corn starch was then modified with sodium borate buffer, casted into a gel and used to run the same DNA sample. Our findings indicated that similar to agarose, the DNA sample and ladder migrated successfully through the modified starch gel but no bands were visible when visualized under blue and ultra-violet light.
Policy change frameworks are commonly used to understand policy development processes. However, few studies have attempted to apply these frameworks to the recent popular fee-free policy education at the high school level in Sub-Saharan Africa. Investigating fee-free policy development through policy change frameworks can assist both in identifying the genesis of past policies, including who the important actors are, how issues are framed and problematised, and how specific solutions are designed, as well as how to interpret unfolding policies. In this article, we review three prominent policy change frameworks: Baumgartner and Jones’ “punctuated equilibrium framework,” Sabatier and Jenkins-Smith’s “advocacy coalition framework,” and Kingdon’s “multiple streams framework.” After reviewing the frameworks, we apply them to two fee-free policies in Ghana which are Progressive Free Senior High School and Free Senior High School policies to understand the drivers of fee-free policy change. From the socio-political background, three main concepts were derived from these policy change frameworks deducing from the basic assumptions of these theories. They are domestic politics, political and policy entrepreneurs, and socio-economic dynamics. The results show that fee-free policies are largely driven by domestic politics and political and policy entrepreneurs in political executive positions. Factors under socio-economic dynamics are only scope conditions that are not significant to trigger the adoption of a fee-free policy.
Governments in Sub-Saharan Africa (SSA) are expanding access to high school education, which is a perpetuation of the previous focus on basic education. This study applies qualitative comparative analysis (QCA) using data from seven countries from 2010 to 2020 to examine the potential conditions of fee-free policies at the high school level. Five potential conditions are analyzed. They include the regime type, electoral competition, ideological lineage, economic conditions, and social context. The findings indicate the significant influence of electoral competition and a high level of lower secondary school enrolment for the adoption of fee-free policies. The absence of electoral competition leads to a lack of fee-free policy. The paper explains how elections, one indicator of representative democracy, motivate political leaders to initiate social policies. Additionally, the study challenges the relevance of two important explanations for expansionary social policy in the literature—the partisan theory of policy outcomes, and the economy.
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