Pycnoporus cinnabarinus (Polyporaceae) is able to produce methylanthranilate in liquid cultures. Study of the culture conditions of P. cinnabarinus IP 1-937 has permitted increase in the aroma productivity by a factor of 16. A low nitrogen concentration, with maltose as carbon source, was required; the culture pH was uncontrolled. The inoculum nature and concentration greatly influence on production: best results were obtained with conidia from a late harvest, used at a rate of 2 x 105 spores/ml. Under these conditions, 18.7 mg methylanthranilate/1 was produced after 5 days of culture. Aroma production is probably connected with the biosynthesis of phenoxazinones, which are characteristic pigments of the genus Pycnoporus.
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