a b s t r a c tGroundwater levels in the North China Plain (NCP), the bread basket of China, have dropped more than one meter per year over the last 40 years, putting at risk the long term productivity of this region. Groundwater decline is most severe in the Alluvial Fans where our study site is located. Avoiding a foreseeable systems collapse requires region-wide changes in crop systems management, underpinned by sound environmental policies. Here, we explore the potential of crop system adaptation to remedy the excessive water use and quantify the likely yield penalties associated with more sustainable water use practices. Using simulations with the APSIM cropping systems model we explore production opportunities in an area within the NCP with intensive cropping and no access to irrigation from rivers. We estimate the attainable production levels for wheat and maize if agriculture were made groundwater neutral, through changes in crop sequence, irrigation practices and water conservation technologies (e.g. mulching with plastic film). Total grain production would drop by 44% compared to current practice if agriculture were made groundwater neutral. Water conservation by plastic film could limit this reduction to 21-33% but possible environmental impacts of plastic film need attention. This analysis facilitates a much needed debate on alternative agronomic practices and incentives through a quantitative comparison of adaptation options. Our biophysical analysis needs to be complemented with socio-economic considerations and discussions with all stakeholders. Similar analyses in other parts of the NCP are possible but require more accurate modelling of landscape hydrology and (towards the coast) risk of salt water intrusion.

The field of long noncoding RNA (lncRNA) research has been rapidly advancing in recent years. Antisense lncRNAs, intergenetic lncRNAs and enhancer lncRNAs can regulate genomic imprinting, which leads to parent-origin-specific monoalletic expression of genes. However, the function of intronic ncRNAs in genomic imprinting remains unclear. Previously, we obtained the cDNA sequence of cattle MEG8 gene, which is located in the DLK1-DIO3 imprinted clusters of cattle chromosome 21. In this study, we undertook a systematic search for transcripts mapping to the MEG8 intronic region and identified three novel lncRNAs, named MEG8 intronic RNA 1 (MEG8-IT1), MEG8 intronic RNA 2 (MEG8-IT2) and MEG8 intronic RNA 3 (MEG8-IT3) according to the GENCODE annotated bibliography. We characterized the expression pattern of these lncRNAs using RT-PCR in adult cattle tissues, and they were expressed in all tested eight tissues, similar to the expression pattern of MEG8. The allele-specific expression of three novel lncRNAs was assessed using a polymorphism-based sequencing approach. Three single nucleotide polymorphism sites were identified in these three lncRNAs. We found that the three lncRNAs showed monoallelic expression in the analyzed tissues, suggesting that they may be imprinted in cattle. These results expand the number of known monoallelically expressed lncRNAs from the DLK1-DIO3 domain and contribute to further investigation of lncRNA regulatory mechanisms and function.