A feeding trial was conducted to study the e¡ect of dietary protein (DP) levels on the growth and digestive enzyme activities of di¡erent wild stocks of Macrobrachium rosenbergii juveniles. Wild juveniles of M. rosenbergii were collected from the west coast of India, Gujarat (G), Maharashtra (M) and from the east coast of India, Andhra Pradesh (A), and raised in culture ponds of 200 m 2 at 1juvenile m À 2 . All the animals were tagged individually with Elastomer tags of a particular colour assigned to their respective stock and acclimatized for 7 days before being released into the pond at a ratio of 70:65:65 (A:M:G). Each of the two feeds, the ¢rst with 27% DP, termed the suboptimum level (S), and the second 32% DP, termed the optimum level (O), was fed in duplicate ponds at 6% of the body mass for the ¢rst 30 days and 4% for the last 30 days. The average weight of stocked prawn, respectively, in O DP and S DP fed ponds was 0.90 AE 0.04 and 1.06 AE 0.08 g for the G stock, 0.80 AE 0.07 and 1.01 AE 0.1g for the M stock and 3.06 AE 0.13 and 3.10 AE 0.23 g for the A stock. Both the protein level and the stock type had a significant (Po0.05) e¡ect on the weight gain% of the prawn. There was an approximate 95% change in weight gain with a DP change. Similarly, G and M stocks exhibited signi¢cantly higher (Po0.05) growth rates of approximately 90% than the A stock, although no di¡erence was noted between the G and the M stocks. However, for protein  stock (interaction) levels, there was no signi¢cant di¡erence (P40.05) among the groups. Although insigni¢cant, the survival rates among the di¡erent stocks varied from 56% to 77%. Optimum protein level showed a signi¢cant increase (Po0.05) in the speci¢c growth rate (SGR). Feed conversion ratio, feed e⁄ciency ratio, protein e⁄ciency ratio and net protein utilization were not a¡ected either due to DP, stock type or their interaction. The O  A group exhibited the maximum variation in body weight. Digestive enzyme activities were similar in all the groups, but enzymes for phospho-monoesterase were signi¢cantly higher (Po0.05) at O DP. Both the G and the M stock showed a signi¢cantly higher (Po0.05) alkaline phosphatase activity while acid phosphatase activity was signi¢cantly higher (Po0.05) in the M stock only. Overall, the G and M stocks showed higher growth responses compared with the A stock.
Material and methods
Experimental animals and taggingWild juveniles of M. rosenbergii were collected from Godavari River, near Rajamundary, in Andhra Pradesh (East coast); Surya River, near Palgarh, in Growth and digestive enzymes of M. rosenbergii juveniles V Sagar et al.