Preoperative preparation of infected wounds of superficial tissues, using the method of hyperbaric oxygenation with the ozone-containing mixture
Objective. To study in experiment the impact of the impulse electrophoresis of the blood plasma method on the wounds healing process in postoperative wounds. Materials and methods. The investigations were conducted on 15 white rats, to which in aseptic environment under intraabdominal narcosis the hip surgical access through the skin and muscles was conducted with subsequent layered closure of the wound, using separate knots. Ten laboratory animals were included into the main group, in whom transdermal introduction of the blood plasma, using the impulse electrophoresis method, was conducted on the 4th, 7th, 10th and 14th postoperative days. Into a control group 5 white rats were included, in which transdermal introduction of the blood plasma was not applied postoperatively. In all the laboratory animals the wounds have healed primarily. On the19th day postoperatively the laboratory animals were extracted from the experiment, and the near suture skin was investigated, using histological methods. Results. The surgical access healing in the hip skin and muscles with formation of normotrophic cicatrices was observed in the main group of the laboratory animals. Hypertrophic cicatrix in the operative intervention zone have been formatted in the laboratory animals of a control group on the 19th postoperative day. Conclusion. More qualitative healing of the wounds with formation of normotrophic cicatrix while application of transdermal introduction of the blood plasma, using the impulse electrophoresis method, was confirmed in experimental investigation in laboratory animals of the main group. Excessive creation of collagen was omitted in the laboratory animals of the main group, what have promoted formation of more thin and tender cicatrix, which have become slightly visible up to the end of experiment.
Objective. Determination of the virus-cidal and bacterio-cidal action of the ozone-containing steam-water mixture, which was obtained, using apparatus POS-1, simulated on viral models of transmissive gastroenteritis of pigs of the coronaviruses family and polyresistant clinical strains of bacterial cultures, as well as adjustment of optimal parameters of ozone in the gaseous mixture content, which do not cause pathological changes in the organism’s organs and systems. Materials and methods. Apparatus POS-1, created by collective of Scientific-Methodical Centre «Medical Innovation Technologies»», was applied for production of the ozone-containing gaseous mixture. Virusological investigations were conducted on the base of the Institute of Epidemiology and Infectious Diseases named after L. V, Gromashevskiy. As a working material the models of virus of the pigs transmissive gastroenteritis of the coronaviruses family were applied. The series of bacteriological and experimental investigations were conducted on the base of Shalimov National Institute of Surgery and Transplantology NAMS of Ukraine. Polyresistant cultures in concentration of 108 colony-creating units per 1 ml were applied for bacteriological investigations. The experiment was conducted on white rats to study the gaseous ozone-containing mixture affection on living biological tissues. Results. Virusological investigations have shown, that in the 20 minutes exposition and a contact with extracellular virus the infection titer have lowered in 100 000 times, and in a 30 minutes exposition - a complete deactivation of virus have had occurred. Bacteriological investigations have revealed the complete absence of the cultures development after their processing with the ozone-containing mixture during 20 min. The results of swimming tests and behavioral reactions in white rats of control and experimental groups did not differ. Histological investigations of the respiratory organs tissues as well as of spleen, thyroid gland, kidneys and suprarenal glands did not reveal pathological effects of the ozone-containing mixture. Conclusion. Parameters of the ozone generation and delivery in the gaseous mixture content were studied and optimally selected. Investigations of the ozone-containing mixture affection on a viral strain of the coronaviruses family, as well as bacteriological investigations on polyresistant bacterial cultures have had confirmed its pronounced virus-cidal and bactericidal properties. Application of the ozone-containing mixture do not cause any pathological changes in the living organism organs and systems. This gaseous mixture may be used for prevention and treatment of respiratory infectious diseases of viral and bacterial genesis.
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