Ungrazed tallgrass prairie plots in the Kansas Flint Hills have been burned annually at 4 different dates since 1928. Time of burning markedly altered the physiognomy and was the crucial factor effecting vegetation change. Late-spring burning, coinciding with emergence of the warm-season perennial grasses, increased grass production and favored Andropogon gerardii and Sorghastrum nutans. Burning in winter, early-spring, or mid-spring reduced herbage production and shifted vegetational composition by differentially favoring other species. Andropogon scoparius increased with mid-and early-spring burning, while perennial forbs and sedges increased with early-spring and winter burning. Amorpha canescens was favored by all burning treatments. Mulch buildup in unburned, undisturbed plots increased Poa pratensis and tree species and eventually reduced grass production. The long-term effects of annual late-spring burning, even in dry years, was not detrimental to herbage production, species composition, or total basal cover in tallgrass prairie.Tallgrass prairie is fire-derived and fire-maintained (Stewart 1951). Historically, fires were intermittent and occurred at nearly any time of the year (Jackson 1965). In the 1880's, cattlemen observed that transient steers gained more weight on burned than on unburned range, and as a result, grazing leases later mandated annual burning (Kollmorgen and Simonett 1965). Time of burning, however, was of little concern, and most pastures were burned in January or February to stimulate earlier greenup.Although voluminous literature exists on vegetational effects from fire, few studies have acknowledged the importance of time of burning. Aldous (1934) initiated preliminary research on burning ungrazed tallgrass prairie at different dates. Subsequent reports on herbage production (McMurphy and Anderson 1963) and botanical composition (McMurphy and Anderson 1965) were nonreplicated, short-term studies in which the unburned control was mowed and raked annually, and included data from years when the plots were not burned. This paper compiles earlier research and adds recent data from undisturbed control plots to evaluate the importance of time of burning on herbage production and species composition in ungrazed tallgrass prairie over the past 56 years. Study Area and MethodsThe experimental site is located on a level ridge top in the Kansas Flint Hills north of Manhattan. From the early 1900's, the area had been moderately grazed by cattle and routinely burned in late winter. Soil at the site is a loessial-derived Smolan silt loam classified as a Pachic Argiustoll. It has a dark-gray surface layer about 20 cm thick overlying a deep silty clay loam subsoil. Vegetation at the beginning of the study was predominantly little bluestem Authors are research assistant and range research scientist, Department of Agronomv. Kansas State Universitv. Manhattan 66506. The voluntarv efforts of Doreen ToLne in manuscript word p&essing are greatly appreciated. . Additional graphs of yearly fluctuations for...

Three experiments were conducted to evaluate effects of supplemental protein vs energy level on dormant forage intake and utilization. In Exp. 1, 16 ruminally cannulated steers were blocked by weight (avg wt = 242 kg) and assigned randomly to a negative control or to one of three isocaloric supplement treatments fed at .4% BW: 1) control, no supplement (NS); 2) 12% CP, low protein (LP); 3) 28% CP, moderate protein (MP); 4) 41% CP, high protein (HP). In Exp. 2 and 3, 16 ruminally cannulated steers were blocked by weight (avg wt = 332 kg, Exp. 2; 401 kg, Exp. 3) and assigned randomly to a 2 x 2 factorial arrangement of treatments. The treatments contrasted low (LP) and high (HP) levels of supplemental protein (.66 g CP/kg BW vs 1.32 g CP/kg BW) with low (LE) and high (HE) levels of supplemental ME (9.2 kcal/kg BW vs 18.4 kcal/kg BW). In Exp. 1, forage DMI as well as ruminal DM and indigestible ADF fill at 4 h postfeeding were greater (P less than .10) with the MP and HP steers than with control and LP steers. Total DM digestibility increased (P less than .10) for supplemented steers (35.5% for control vs 47.3 for supplemented steers); however, LP depressed (P less than .10) NDF digestibility. In Exp. 2, forage DMI, indigestible ADF flow and liquid flow were depressed (P less than .10) in LP-HE supplemented steers. In Exp. 3, HP steers had greater (P less than .10) forage DMI, indigestible ADF fill values (4 h postfeeding), liquid volume and tended (P = .11) to have greater ruminal DM fill (4 h postfeeding). In summary, increased levels of supplemental protein increased intake and utilization of dormant tallgrass-prairie forage (less than 3% CP). Increasing supplemental energy without adequate protein availability was associated with depressed intake and digestibility.

Ruminal samples were collected at slaughter from 364 unfasted steers fed different finishing diets to obtain information on numbers and species distribution of ciliated protozoa in feedlot cattle. Total numbers of protozoa averaged 1.59 X 10(5)/g of ruminal contents. A total of 47 steers (12.9%) were defaunated, but 4.1% of the steers possessed numbers of protozoa greater than 10(6)/g. Entodinium species did not always dominate the protozoan populations; 41 faunated steers (11.2%) were devoid of entodinia, and 79 additional steers (21.7%) possessed populations dominated (greater than 50%) by other genera. Isotricha was the most commonly occurring genus supplanting Entodinium, but Polyplastron and Epidinium were frequently present in high concentrations. Tallow and soybean soapstock supplementation reduced (P less than .05) numbers of protozoa in steers consuming wheat diets. However, yellow grease supplementation did not affect numbers of protozoa in steers fed either sorghum or corn diets. Average ruminal pH was 6.20 on the wheat diet, 6.05 on the corn diet, and 5.69 and 6.23 for the two sorghum diets, respectively. We found no correlation between ruminal pH and numbers of protozoa on any diet. The presence of relatively high protozoan concentrations and few defaunated animals in feedlot cattle necessitates reevaluation of the role that ciliated protozoa play in ruminal metabolism of animals fed processed, high-concentrate diets.

The objective of this study was to assess the influence of ciliated protozoa on ruminal fermentation in cattle fed high-grain diets. Six ruminally cannulated steers fed a corn-based grain diet (85% concentrate plus 15% alfalfa hay) at 12-h intervals were assigned randomly to two groups, ciliate free and faunated, in a crossover design. Defaunation was by ruminal emptying, omasal flushing, and treatment with sodium sulfosuccinate. Two to 3 weeks after defaunation, the ruminal contents of all steers were sampled before the morning feeding (0 h) and at 1, 2, 4, 6, 8, and 12 h after feeding to measure pH, analyze fermentation products, and monitor counts of ciliated protozoa and lactic acid-producing and-fermenting bacterial groups. Total numbers of ciliated protozoa in the faunated steers averaged 4.3 x 105/g, and the protozoa consisted of nine genera. Ciliate-free steers had lower (P < 0.01) ruminal pHs (pH 5.97) than faunated cattle (pH 6.45); however, the treatment-time interaction was not significant. Ruminal lactate and ammonia concentrations were similar in both groups. The total volatile fatty acid concentration was higher (P < 0.05) in the ciliate-free steers than in the faunated steers and exhibited a treatment-time interaction (P < 0.05). The acetate-to-propionate ratio was higher (P < 0.05) in the faunated group than in the ciliate-free group and showed a treatment-time interaction (P < 0.05). Total anaerobic bacterial counts were about fourfold higher in the ciliate-free group than in the faunated group. Although counts of lactic acid producers were higher and counts of lactic acid fermenters were lower in ciliate-free steers than in faunated steers, the differences were not significant. Ciliated protozoa in cattle fed high-grain diets apparently moderate the ruminal fermentation rate as evidenced by higher ruminal pH values and lower volatile fatty acid concentrations in faunated cattle than in ciliate-free cattle. It appeared that the moderation of the ruminal fermentation rate by ciliated protozoa was attributable to reduced bacterial numbers and possibly reduced activity. * Corresponding author. t Contribution no. 92-491-J from the Kansas Agricultural Experiment Station.

Ruminal microbial populations, fermentation characteristics, digestibility, and liquid flow rates in two ruminally cannulated bison and two ruminally cannulated Hereford steers fed a prairie hay diet were compared. No significant differences in anaerobic bacterial counts, volatile fatty acid concentrations, or ruminal pHs were evident between bison and cattle. Also, no significant differences in neutral detergent fiber digestibility, indigestible fiber retention time, or intake were detected between bison and cattle, although cattle had higher levels (P < 0.08) of ruminal dry matter and indigestible fiber than bison. Bison had a smaller (P = 0.02) ruminoreticular volume, faster liquid dilution rates, and faster liquid turnover times than cattle. The average ruminal ammonia nitrogen concentration was higher (P = 0.02) in bison (1.17 mg/dl) than in cattle (0.79 mg/dl). Total ciliate protozoal counts and cell volume were greater (P = 0.07) in bison (32.8 x 104/g and 407.1 x 10-4ml/g, respectively) than in cattle (15.7 x 104/g and 162.2 x 10-4 ml/g, respectively). Bison harbored higher (P < 0.02) numbers of Dasytricha spp., Eudiplodinium maggii, Eudiplodinium bursa, and Epidinium spp. than cattle and possessed a type B protozoan population. The cattle possessed a mixed type A-type B population that was characterized by Ophryoscolex spp. and Polyplastron spp. in association with low concentrations of Epidinium spp. and Eudiplodinium maggii. * Corresponding author. t Contribution no. 88-472-J of the Kansas State Agriculture Experiment Station, Manhattan.