A new procedure for fast quantitative extraction of cell parameters from diffraction patterns was deduced from model calculations and applied to cervical gynecological material. Based on radial scans of the diffraction pattern, the technique permits simultaneous determination of the nuclear and cytoplasmic diameters by Fourier analysis of the radial scanning signals after compensation for the intensity falloff by an amplification proportional to the third power of the radial position in the diffraction plane. Illustrative examples of measurements on exfoliated cells of different types are presented.
Measurements of tile absorption coefficient and the refractive index of crystalline lithium fluoride have been carried out in the infrared region between 300 and 900 microns wavelength. The spectrometer which has been used was of the periodic interference-modulator type. Some general properties of this instrument are described.
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