By column chromatography on a column of polyamide sorbent (elution with 40% ethanol) we have isolated from the leaves of Sorbaria sorbifolium (Ural falsespirea) in the Khekhtsirskii reserve (Kharabov territory) a substance (I)of flavonoid nature, C21H~O20 • H20 with mp 218-220" C, kma x g35, 286 m/£ The hydrolysis of substance I with 10% sulfuric acid in the presence of acetic acid yielded an aglycone which, from its melting point, Rf value and the absence of a depression of the melting point in a mixture with an authentic sample, was identified as sentellarein. Glucuronic acid was detected in the mother liquor after the separation of the aglyeone and neutralization with alkali to pH 5 by paper chromatography.On the basis of the constants given, the products of hydrolysis, and a direct comparison with an authentic sample, substance I was identified as scutellarin (scutellarein 7-glyeuronide) [1].The sample of scutellarin was obtained from V. A. Bandyukova (Pyatigorsk).
REFERENCEBy the separation on a Kapron column of a purified ethanolic extract of the herb Hedysarum neglectum we have isolated four individual phenolic compounds.The first substance was identified as the xanthone derivative mangiferin (hedysaride) [1].On the basis of their physieochemical constants, the products of acid and enzymatic hydrolysis, their IR and UV spectra with ionizing and complex-forming agents, and a comparison of the molecular rotations of glycosides with those of the corresponding phenyl glycosides, the other substances were identified as polystachoside (quercetin 8-~-L-arabofuranoside) [2], hyperoside [3], and quercetin 3-cz-L-rhamnofuranoside [4].
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