Microscopical.-On three separate occasions, the first on the fourteenth day, the urine was examined for spirochaetes, and these were found. The specimens were centrifuged, and the deposit examined under dark-ground illuminatIon, and also stained by Fontana's silver method.It was found necessary to obtain a very fresh specimen in order to observe the movements, which consisted of rotation and undulation. In stained preparations the spirochaetes were not very numerous, but about a dozen or so of these were seen, and one (the first) preparat,on was photographed. There was a distinct hook-like tendency at the termination of the spirochaete, but the textbook well-marked hooking was only obvious in a few specimens. It must be remembered that the urine has an action on the morphological appearances, and tends to make the organism appear somewhat atyp:cal.Biological.-Rather late in the disease animal inoculation of the centrifuged deposit was tried, and a guinea-pig was injected intraperitoneally. No effects were observed, the animal being killed a fortnight after injection.A. D. Gardner' states that the organism occurs both in fresh and in sea water, and can be cultivated therefrom.Dr. R. A. O'Brien very kindly consented to test to serological agglutination against the leptospira with a specimen of the patient's serum, but suitable strains are not for the moment available.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.