Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has proved to powerful research tool due to its sensitivity, high selectivity, and high throughput efficiency..Sirolimus was extracted from plasma by two-step extraction procedure using chloroform as extracting solvent. Signal intensity was high using ESI(+) source provided for the quantitation of samples. Chromatographic separation was performed on phenomenax C-18 column (250 x 4.60 mm 5microns).Mobile phase contains acetonitrile, water (80; 20 v/v) + 0.1% acetic acid, flow rate 1 mL/min.The retention time of Sirolimus 8.4 min, the total run time10 min. Linearity correlation coefficients (r(2)) curve was 0.997183.calibraction range 10-1000 ng/mL. The UV detection of Sirolimus was at 278(277.78) nm. Sirolimus coated drug eluting stents, MRM (Multiple reaction monitoring) transition of Sirolimus m/z 936.83-208.84 was selected to obtain maximum sensitivity. LC/MS/MS results exhibited consistency in drug content on the stent surface. In-vitro release kinetic indicated the release of Sirolimus in 41 days from the date of implanted. Drug release was found at the first day, burst release was observed at 7(th) day of implantation. This study involved pharmacological coating of stents, based on the notion that sustained systemic local delivery of anti-proliferative agents. LC-MS/MS method has been successfully used in the pharmacokinetic analysis of Sirolimus coated drug eluting stents.
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of the Paclitaxel in human plasma was developed and validated.Placitaxel was extracted from plasma by a two-step extraction procedure with using of chloroform as Liquid-Liquid extractive organic solvent. LC-MS/MS analysis using electro-spray ionization (ESI+) was performed on Phenomenex C-18 Column (250x1.5μ) using as acetonitrile, water (80:20+0.1% acetic acid) as mobile phase. The method has a fl ow rate of 0.8ml/min. Retention of Paclitaxel was 4.60 minutes.An excellent linearity (r 2 .099) between the peak ratios and Paclitaxel concentrations over the range of 10-100 ng/ml of plasma was studied. The lower limit of detection for Paclitaxel on mass was 10 pg/mL. There was about 100 percent of Paclitaxel was recovered in extracted samples. The study of Paclitaxel standard and extraction standard calibration curves were useful in pharmacokinetics analysis.
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