depended on FEV, (% predicted) more or less than 75%. Chrispin-Norman scores were calculated on all patients, whereby a lateral chest radiograph and the four quadrants of the lung fields on a posterior/anterior film were scored with regard to chest configuration and lung markings; a high score indicated a poor chest radiograph (maximum 38 points).9Colonisation with Pseudomonas species was noted; patients were scored as positive if the organism was isolated even once, even though the infection was apparently not persistent. All parameters were assessed during the year before the analyses. GENOTYPE ANALYSES Data were sought for nine different mutations: AF508, AI507, 1717-IG>A, G551D, R553X, G542X, R560T, R117H, and 621 + IG> T. The AF508 deletion was identified by amplification of genomic DNA by polymerase chain reaction (PCR), followed by direct measurement of the size of the PCR product on a denaturing polyacrylamide gel. Multiplex PCR was used in some patients to screen simultaneously for five mutations (J P Cheadle, personal communication
It was a privilege to be invited to open the series of reflections on the NHS at the 50th Anniversary Celebration at Westminster Abbey. My title was The vision of dignity and the challenge - respect and care for all'.
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