An egg-weight-depressing factor in fababeans was isolated in pure form from a white precipitate which had been shown in a previous study, to contain a concentrated form of this factor. The analytically pure compound was obtained in colourless, needle crystals. Its melting point was 242-244"C, it contained 17.4% nitrogen but did not react with ninhydrin. The spectra had adsorption maxima at 274, 276 and 269 nm in acidic, neutral, and alkaline solutions, respectively. These and other properties were identical to those of vicine [2,6-diamino-4,5-dihydroxy pyrimidine,5-( ,9-~-glucopyranoside)] a compound known to be present in fababeans. Vicine and convicine [2,4,5-trihydroxy, 6-aminopyrimidine,5-(,9-~-g~ucopyranoside)]; compounds having similar chemical and probably physiological activity, were resolved on a cationexchange column and were detected by monitoring at 280 nm. Quantitative results obtained with the procedure was shown to be similar to those obtained with two other procedures. This method, however, was superior to other methods as it gave individual values for both vicine and convicine rather than a composite value; sample preparation was simple and the method could readily be adapted to automatic analysis.
Sci. 50: 395-'105 (June 1980) and Teague (1914) found that 2OVo fababeans in laying hens diets had no effect on egg production or feed consumption but resulted in a significant decrease in egg size.In contrast, Davidson (1973)
Two experiments were conducted to study the egg weight depressing effect of an ethanol-water extract of autoclaved fababean protein concentrate. Acetone fractionation of the extract produced four fractions, one of which (fraction H ) , significantly depressed egg weight. Further fractionation of fraction H produced two fractions; a highly water-soluble supernatant fraction and a fraction with low water solubility which was harvested as a white precipitate. Both the supernatant and the white precipitate, when added to diets in proportion to their relative yields, caused similar depressions in egg weight. The observation that there was a direct relationship between amounts of vicine-reactive material in the different fractions and the magnitude of egg weight depression indicates that the principal egg weight depressing factor in fababeans is vicine and/or convincine.
bcan fractions on cgg weight among laying hcns fed test diets for a short time period. Can. J. Anim. A short,term bioassay test using Single Comb White Leghorn (SCWL) laying hens was cleveloped to study various tiactions of fababeans. to determine the location of the egg-weight-depressing factor ( L,wDF) , and to determine the cff'ect of hcat treatment on EWDF activity. A second objcctive of the research was to develop an extraction method to facilitate studics on idcntification and mode of action of the factor(s). The t4-day pcriod proved useful in confirming that EWDF activity was concentrated in the cotyledon portion offababean seeds and associatcd with thc protein rather than the starch component. Ethanol-watcr extraction was cfTectivc in conccntrating the factor' Un dosage biologique de coufte dur6e utilisant des pondeuses Leghorn ) cr6te simple a 6t6 mis au point pour dutdier les diverses fractions de la f'6vcrole et ainsi ddterminer l'emplacement du factcur de r6duction du poids de I'oeuf (FRPO)
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