Bioactive hydroxyapatite (HA) with addition of silicon (Si) in the crystal structure (silicondoped hydroxyapatite (SiHA)) has become a highly attractive alternative to conventional HA in bone replacement owing to the significant improvement in the in vivo bioactivity and osteoconductivity. Nanometre-scaled SiHA (nanoSiHA), which closely resembles the size of bone mineral, has been synthesized in this study. Thus, the silicon addition provides an extra chemical cue to stimulate and enhance bone formation for new generation coatings, and the next stage in metallic implantation design is to further improve cellular adhesion and proliferation by control of cell alignment. Topography has been found to provide a powerful set of signals for cells and form contact guidance. Using the recently developed novel technique of template-assisted electrohydrodynamic atomization (TAEA), patterns of pillars and tracks of various dimensions of nanoSiHA were achieved. Modifying the parameters of TAEA, the resolution of pattern structures was controlled, enabling the topography of a substrate to be modified accordingly. Spray time, flow rate and distance between the needle and substrate were varied to improve the pattern formation of pillars and tracks. The 15 min deposition time provided the most consistent patterned topography with a distance of 50 mm and flow rate of 4 ml min 21. A titanium substrate was patterned with pillars and tracks of varying widths, line lengths and distances under the optimized TAEA processing condition. A fast bone-like apatite formation rate was found on nanoSiHA after immersion in simulated body fluid, thus demonstrating its high in vitro bioactivity. Primary human osteoblast (HOB) cells responded to SiHA patterns by stretching of the filopodia between track and pillar, attaching to the apex of the pillar pattern and stretching between two. HOB cells responded to the track pattern by elongating along and between the track, and the length of HOB cells was proportional to the gaps between track patterns, but this relationship was not observed on the pillar patterns. The study has therefore provided an insight for future design of next generation implant surfaces to control and guide cellular responses, while TAEA patterning provides a controllable technique to provide topography to medical implants.
Hydroxyapatite (HA)-coated metallic prostheses, which combine the osteoconductivity of HA and high strength of metallic alloys, have been increasingly the choice of joint replacement prostheses by surgeons as the general population lives longer. Surface modification of metallic implant surfaces is one of the key focal points to implantation technology. In addition to material chemistry, surface topography has been found to positively impact cellular response and is able to enhance the life time of the implant. Recently, a new technique, template-assisted electrohydrodynamic atomization (TAEA) spraying, developed using the principles of electrohydrodynamic atomization spraying, which is an electrically driven jet-based deposition method, is of considerable interest in surface topography formation. The process offers the attractive advantages of compatibility with micro-fabrication technology and versatility in pattern specification for advanced implant designs. This technology incorporates nanosized calcium phosphate to mimic the size and chemical composition of bone mineral in a micrometer-dimension pattern configuration to guide cellular responses. In vitro studies showed that both pillar and track nano Silicon-substituted HA (SiHA) patterns were able to encourage the attachment and growth of osteoblast cells, the track patterns provided the favourite surface for the initial cell attachment while a fast cell proliferation rate was found on the pillar pattern from day 1 to day 5 in comparison with that of a SiHA-coated surface. The alignment of actin cytoskeleton of osteoblast cells matched the orientation of the entire cell. The shear peel strength of the patterned interlocking nano-HA coating was found to be at least an order of magnitude higher than the conventional HA coating. Therefore, TAEA offers great potential for producing new coatings with a tailored surface topography, on both the micro- and nano-scale in a more cost effective way to enhance the performance of medical implants.
Template-assisted electrohydrodynamic atomization (TAEA) spraying deposition, a recently developed and an electric-driven jet-based technique has been used to prepare bioactive surface topography on titanium (Ti). Nanometer-scaled SiHA (nanoSiHA), which closely resembles the bone mineral, has been synthesized and deposited on Ti surfaces with a range of patterns, such as pillars and tracks. A human osteoblast (HOB) cell model has been used to evaluate the in vitro cellular responses to nanoSiHA deposition. alamarBlue™ assay showed that nanoSiHA patterns are able to encourage the attachment and growth of HOB cells in comparison to those of nanoSiHA coating. The preferential growth of HOB cells was found along and across the track, HOB cells were also found to stretch between two tracks. Image analysis of HOB cell responses to the size of nanoSiHA pattern showed that the length of HOB cells was proportional to the gaps between the tracks until reaching the maximum length of 110 μm. The results indicate that the distance between the structures is paramount over the width. Our study will pave the way to control and guide cellular responses for new generation of bone implants.
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