We first established a human ovarian sarcomatoid carcinoma cell line designated BUPH:OVSC from primary culture. The specimen was derived from the mural nodule in an ovarian mucinous tumor and cultured in vitro. To date, the cell line has been maintained for over 100 passages. Its biologic characteristics were studied by light and electron microscopy, which revealed spindle-shaped or polygonal cells with a doubling time of 39.5 h. The agglutination test of BUPH:OVSC was positive, and cell colonies were formed in soft agar. Chromosome analysis revealed its karyotype to be a pseudodiploidy. One X chromosome deletion and chromosome 20 addition were detected, and aberrant chromosomes t (1q;12q) and 14p(+) were its chromosome markers. BUPH:OVSC was tumorigenic in nude mice. Hematoxylin and eosin staining of transplanted tumors showed that the cells were morphologically sarcomatoid. However, the transmission electron microscopic observation exposed its epithelial origin. The cell line coexpresses cytokeratin and vimentin. It dose not appear to express estrogen and progesterone receptors or the CA125 tumor marker. Alcian blue/periodic acid-Schiff staining indicates that the cells could secrete acid mucopolysaccharide. In conclusion, BUPH:OVSC displays unique cellular properties, which make it a useful model for the study of human ovarian sarcomatoid carcinomas.
Background: There were a lot of tumor-associated proteins before. Maspin is a tumor-suppressor protein and its prognostic value in lung adenocarcinoma has been reported. However, little is known about the clinical impact of subcellular localization of maspin in early-stage lung adenocarcinoma. We aimed to evaluate the clinical significance of subcellular localization of maspin in patients with pathological stage (pstage) IA lung adenocarcinoma patients categorized by the new eighth edition TNM classification. Method: We immunohistochemically analyzed 181 tissue samples from p-stage IA1 (n¼37), IA2 (n¼92) and IA3 (n¼52) lung adenocarcinomas using antibody for maspin. Result: The 181 cases fell into five predominant subtypes: lepidic (n¼32), acinar (n¼97), papillary (n¼30), solid (n¼20) and micropapillary (n¼2). The frequencies of maspin staining were: cytoplasmic-only in 24.9%; pancellular (nuclear and cytoplasmic) in 8.8%; nuclear-only in 0.6%; no staining in 65.7%. Cytoplasmic-only staining significantly correlated with high pathological T-classification (p¼0.039), lymphatic invasion (p¼0.002) and poorer tumor differentiation (p¼0.002). The patients were followed up for 12-151 months (median¼74 months), and the cytoplasmic-only staining significantly correlated with shorter disease-free survival (DFS) (p¼0.034) and disease-specific survival (DSS) (p¼0.036) by log-rank tests. In Cox's multivariate analysis, lymphatic invasion had the most significant effect on shorter DFS and DSS. Conclusion: The expression of maspin in the cytoplasm alone could be useful for predicting unfavorable prognoses in patients with pstage IA lung adenocarcinoma.
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