The fluorogenic model substrates, methylumbelliferyl [MUF]-/3-D-glucoside, MUF-phosphate and MUF-sulphate, were used to investigate the activities of/3-glucosidase, phosphatase and sulphatase, respectively, in Welsh peatland soils. The method was used to investigate depth dependent variations in enzyme activity in a riparian wetland, and flush channel wetland. The highest activities were found at depths of less than 10 cm, thus confirming other studies which suggest this upper depth to be the site of greatest microbial activity. The most serious limitation to the technique was found to be the (fluorescence) quenching effects of the phenolic materials that dominate peatland dissolved organic carbon. The problem necessitates the adoption of a time consuming quench correction procedure with every sample. Fluorogenic substrates have led to a greater understanding of the role of enzymes in other aquatic systems. It seems likely that they will prove of equal value in elucidating their role in nutrient cycling and the biogeochemistry of peatlands.
Microbial activity and enzymic decomposition processes were followed during a field-based experimental lowering of the water table in a Welsh peatland. Respiration was not significantly affected by the treatment. However, the enzymes sulphatase,/3-glucosidase and phosphatase were stimulated by between 31 and 67% upon water table drawdown. A further enzyme, phenol oxidase, was not significantly affected. The observation of elevated enzyme activities without an associated increase in microbial respiratory activity suggests that drought conditions influence peatland mineralisation rates through a direct stimulation of existing enzymes, rather than through a generalised stimulation of microbial metabolism (with associated de-novo enzyme synthesis). Hydrochemical data suggest that the stimulation may have been caused by a reduction in the inhibitory action of iron and phenolics in the peat pore waters. Overall, the findings support the recent hypothesis that drier conditions associated with climate change could stimulate mineralisation within wetlands.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.