Aims: Avermectins are major antiparasitic agents used commercially in animal health, agriculture and human infections. To improve the fermentation efficiency of avermectins, for the first time a plasma jet generated by a novel atmospheric pressure glow discharge (APGD) was employed to generate mutations in Streptomyces avermitilis.
Methods and Results: The APGD plasma jet, driven by a radio frequency (RF) power supply with water‐cooled and bare‐metallic electrodes, was used as a new mutation method to treat the spores of S. avermitilis. The plasma jet yielded high total (over 30%) and positive (about 21%) mutation rates on S. avermitilis, and a mutated strain, designated as G1‐1 with high productivity of avermectin B1a and genetic stability, was obtained.
Conclusions: Because of the low jet temperature, the high concentrations of the chemically reactive species and the flexibility of its operation, the RF APGD plasma jet has a strong mutagenic effect on S. avermitilis.
Significance and Impact of the Study: This is a proof‐of‐concept study for the use of an RF APGD plasma jet for inducing mutations in microbes. We have shown that the RF APGD plasma jet could be developed as a promising and convenient mutation tool for the fermentation industry and for use in biotechnology research.
We report structural and physical properties of the single crystalline
. The X-ray diffraction (XRD) results show that
adopts the trigonal
-type structure. Temperature-dependent electrical resistivity
measurements indicate an insulating ground state for
with activation energies of 40 meV and 0.64 meV for two distinct regions, respectively. Magnetization measurements show no apparent magnetic phase transition under 400 K. Different from other
, Sr, and Ba, and
, As, and Sb) compounds with the same structure, heat capacity
and
reveal that
has a first-order transition at
and the transition temperature shifts to high temperature upon increasing pressure. The emergence of plenty of new Raman modes below the transition, clearly suggests a change in symmetry accompanying the transition. The combination of the structural, transport, thermal and magnetic measurements points to an unusual origin of the transition.
It is interesting and of significant importance to investigate how network structures co-evolve with opinions. The existing models of such co-evolution typically predict that network nodes either reach a global consensus or break into separated communities, each of which has its own community consensus. Such results, however, cannot explain the richness of real-life opinions, which are typically diversified with no global or even community consensus. In addition, people seldom, if not never, totally cut themselves off from dissenters. In this article, we show that, a simple model integrating consensus formation, link rewiring and opinion change allows complex system dynamics to emerge, driving the system into a dynamic equilibrium with co-existence of diversified opinions. Specifically, similar opinion holders may form into communities yet with no strict community consensus; and rather than being separated into disconnected communities, different communities are connected by a non-trivial proportion of inter-community links. More importantly, we show that the complex dynamics may lead to different numbers of communities at steady state with a given tolerance between different opinion holders. We construct a framework for theoretically analyzing the coevolution process. Theoretical analysis and extensive simulation results reveal some useful insights into the complex co-evolution process, including the formation of dynamic equilibrium, the phase transition between different steady states with different numbers of communities, and the dynamics between opinion distribution and network modularity.
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