A series of macrocyclic ligand complexes of cobalt(II) having bromides in the axial position and containing one or two hydroxyl groups have been synthesized from either biacetyl or benzil and 1,3-propanediamine and l,3-diamino-2-hydroxypropane. By adding one diamine and the diketone followed by Co(II) and the second diamine yields of the unsymmetrical macrocyclic ligands containing one hydroxyl were obtained rather than a statistical distribution of ligands containing the two diamines. A macrocycle precursor from condensation of two benzils with one 1,3-propanediamine was prepared and isolated. This precursor would only react further with a second diamine if Co(II) is present, demonstrating the template nature of the reaction.
A lambda gt 11 cDNA library, constructed from poly(A)+ mRNA isolated from Avena fatua aleurone layers incubated with 1 microM gibberellin A1 (GA1) for 4 days, was screened with an anti-idiotypic antiserum raised against the GA-specific monoclonal antibody MAC 182. One positive clone was isolated, sequenced and shown to encode a tetraubiquitin based on the deduced amino acid sequence. This polyubiquitin cDNA exhibited a high degree of homology to a cloned wheat hexaubiquitin in its 3'-non-coding region. Analysis of total RNA isolated from A. fatua aleurone layers, treated without or with a range of concentrations of GA1 from 10(-11) to 10(-6) M, by northern blotting using the cDNA probe revealed 8 different ubiquitin-containing transcript classes all of which are constitutively expressed in aleurone and are regulated by GA1.
Durch Reaktion von Diacetyl oder Benzil mit 1,3‐Diaminopropan und/oder seinem 2‐Hydroxyderivat in Methanol in Gegenwart von HBr und Co(II)‐Ionen′werden die Komplexe (I) dargestellt.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.