Aherdeen ABY IAS atid $ Woodetid Hospitul. Aherdeeti ABYZYS, U.K.Plasma high-density lipoprotein (HDL) contains several structurally and functionally distinct subfractions, the distribution of which is different in subjects of high and low coronary risk [ 11. It has been suggested that specific subfractions, rich in apolipoprotein E, are implicated in the efflux of cholesterol from artery walls [2] and thereby confer the protective effect of HDL against coronary heart disease. Since this disorder is the major cause of death in the elderly, it is important to know whether the normal plasma concentrations of HDL subfractions and other lipoprotein components that have been measured in younger subjects are applicable to the elderly.Blood samples were collected from 30 male and 45 female subjects, aged between 6 2 and 94 years, and also from eight male and 21 female subjects of the same age range with Alzheimer's disease. None of the subjects showed evidence of myocardial infarction or peripheral vascular or cerebrovascular disease. Very-low-density lipoproteins, low-density lipoproteins (LDL) and HDL were separated from the plasma by flotation in the preparative ultracentrifuge as previously described [ 31. Lipoprotein and total plasma cholesterol were determined by enzyme assay. HDL subfractions were separated by electrophoresis on Pharmacia 4/30 polyacrylamide gradient gels and the composition determined by scanning using the Bio-Rad model 620 video densitometer
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