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Medial sesamoid bones from the metacarpophalangeal joints of calves were used for prolonged culture of anatomically intact articular cartilage on its natural bone support. The cartilage remained viable during culture, without signs of degeneration. After 1 wk of culture the cartilage showed an increased proteoglycan synthesis, and some minor changes in the composition of newly synthesized proteoglycans were observed. In the next 7 wk all studied parameters remained constant, except for the rate of proteoglycan synthesis, which declined between 4 and 8 wk to values just below those measured at the start of culture. Despite the fact that newly synthesized proteoglycans showed some altered biochemical properties, the composition of the total pool of proteoglycans did not change during 8 wk of culture. The significance of this phenomenon is discussed. This new in vitro model of intact articular cartilage offers a promising alternative to in vivo studies because in contrast to other in vitro models no surgical injury of the cartilage is introduced.

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