123 Genet Resour Crop Evol (2011) 58:125-137 DOI 10.1007/s10722 -010-9601-5 currently consists of 220 accessions from 15 countries: 169 of these come from European cultivation countries, 18 from commercial areas in non EU countries, 26 from regions of minimal or relict production and/or from abandoned fields and 7 from commercial nurseries. The non-saffron Crocus collection currently comprises 352 accessions: 179 collected from the wild in 12 countries of natural distribution, 24 from donations of public and private institutions, 91 from commercial nurseries and 58 acquired from BGV-CU collection management. Here we provide a record of collections, activities concerns and current strategies for documentation, conservation, characterisation, and management of the collection as important tools for researchers with interest in these valuable genetic resources.
A previously reported cytoplasmic male sterilityassociated fragment was detected in chili pepper (Capsicum annuum L.) and sequenced in the mitochondria of a sterile plant. This fragment, 16 kb in size, contains two common mitochondrial genes, coxII and atp6-2, and the newly detected gene orf456, which appears to be a strong candidate gene for the male sterile phenotype. The data show that one nucleotide (Cytosine) at the +449 position is missing compared with the original sequence data of orf456. By translation to the amino acid sequence, the termination codon was found 49 nt downstream from the original site. This hypothetical extension was confirmed by RT-PCR, which showed a sequence size of 507 bp. Since pollen fertility can be restored by a nuclear restorer of fertility gene (Rf), the transcription of orf507 was checked in four stages of flower development. The RT-PCR resulted in a fragment in stage III in the restorer line, indicating a possible role in microspore development.
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