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Due to its unique, unanimously recognized properties and biocompatibility, graphene has wide potential applications in biology, biomedical science, environmental agriculture and biotechnology. The antibacterial effect of the graphene is presented in a large number of publications. Most studies reported in the specific literature were aimed mainly at understanding the interaction between graphene and graphene-based materials with cells and bacteria. Even so, there are conflicting results in some cases and there are also numerous controversies regarding the antibacterial effect of monolayer graphene film on different types of substrate.The study is aimed at testing the antibacterial activity of monolayer graphene film on a copper substrate that was covered with a Staphylococcus aureus culture, Gram-positive bacteria recognized for resilience in external environment. The antibacterial activity of the graphene was evaluated via cell-viability test. It has thus been observed that the bacterial suspension's phisical contact with the a large-area graphene produces significant disturbances of the microorganism's vital processes.This study may provide new insights for the better understanding of antibacterial actions of graphene applied on different substrates and opportunities for biomedical applications.

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The electrocardiogram (ECG) is a graphical recording of the cardiac electric activity during cardiac revolutions. This bio-current triggers and maintains the mechanical activity of the heart. Within this research, the amplitudes values of the electrocardiographic waves were determined in 6 leads: I, II, III, aVL, aVR and aVF. Thus, some electrocardiograms were recorded using limb lead in clinically healthy kids, aged 1 month, 3 months and 5 months, in order to achieve the proposed objectives. Then, the statistical analysis of the obtained results was performed using t (student) test.As a consequence of the interpretation of the obtained results, it was noticed that the limb leads can be used successfully for recording the electrocardiogram in kids because it provides an easy aspect to interpret. The highest amplitude of the electrocardiographic waves is recorded in I lead, in the case of the 1 month old kids (having the following values: 0.115 mV ± 0.010 for P wave; 0.625 mV ± 0.078 for QRS complex; 0.460 mV ± 0.045 for T wave) and in II lead (having the following values for the 3 months old kids: 0.071 mV ± 0.015 for P wave; 0.540 mV ± 0.064 for QRS complex; 0.310 mV ± 0.052 for T wave and having the following values for the 5 months old kids: 0.071 mV ± 0.015 for P wave; 0.455 mV ± 0.028 for QRS complex; 0.430 mV ± 0.026 for T wave). It also found that the lowest amplitude of electrocardiographic waves is recorded in the aVF lead, but this lead cannot be used for ECG recording in kids.