Comparative microspectrophotometric study of the nuclear Feulgen DNA content in pretumorous processes and cancers (larynx, endometrium, large intestine, and stomach) has made it possible to formulate an exponential law of DNA accumulation in cell populations in proliferating and malignant tissue. The dynamics of changes in the "index of DNA accumulation" can be used as an objective quantitative diagnostic test. A scale of malignant transformation was proposed with which the degree of probability of malignancy in pretumorous processes could be estimated. The data obtained allowed the author to propose a hypothesis of DNA superproduction as the basic cause of progressive neoplastic growth.
Clinical signs of aging verified by morphometrical analysis of brain tissue were observed in young mice 4 months after administration of brain extract from old mice (5 intraperitoneal injections).
Comparative computer-assisted microspectrophotometric analysis of histological preparations showed that the mean nuclear ploidy increased 1.3 times in glandular hyperplasia and 2-fold in adenocarcinoma of 3 progressive degrees of dysdifferentiation. Proliferative activity of cells also increased from stage to stage. The data visualize cell nuclei ploidy and facilitate objective differential diagnostic decision making.
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