A polymerase chain reaction assay was developed for detection of Fusarium sporotrichioides, a plant pathogen in many parts of the world. Based on small nucleotide differences in ITS2 (Internal Transcribed Spacer) rDNA of our local isolate of F. sporotrichioides (Accession No. AY510069) and other isolates found in NCBI/GeneBank database, species specific primer FspITS2K was selected. Primer pair FspITS2K and P28SL amplified a fragment of 288 bp containing a portion of ITS2 and 28S rDNA of all the F. sporotrichioides isolates tested, originated from different hosts and regions of the world but did not amplify any other species of Fusarium and plant's DNA. To use the PCR assay in seed health testing, a protocol was setup for the rapid and effective preparations of fungal DNA from wheat seeds. The method developed may be useful for the rapid detection and identification of F. sporotrichioides both from culture and from plant tissue.
Information on seed physiology and biochemistry is presented under the following headings: the legume seed, including seed components and seed development; the accumulation and biosynthesis of carbohydrates, including accumulation of soluble carbohydrates (monosaccharides, disaccharides and oligosaccharides), biosynthesis of soluble carbohydrates (raffinose family of oligosaccharides, cyclitols and galactosyl cyclitols), accumulation of starch and biochemistry of starch; and physiological role of carbohydrates in legumes seeds, including during seed development (zygotic seeds, somatic embryos and mechanism of desiccation tolerance), during temperature stress, during seed storage and during germination (degradation of starch, degradation of raffinose family of oligosaccharides and the loss of desiccation tolerance).
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