A study was conducted to evaluate the nutrient content and variability of distiller's dried grains with solubles (DDGS) originating from new (less than 5 yr old) ethanol plants in Minnesota and South Dakota. Ten plants (8 MN, 2 SD) participated in the study, submitting a total of 118 samples. Samples were collected every 2 mo from ten ethanol plants in the Minnesota-South Dakota (MNSD) region from 1997 to 1999 and were analyzed for amino acid levels, DM, CP, crude fiber, crude fat, ash, ADF, NDF, Ca, P, K, Mg, S, Na, Zn, Mn, Cu, and Fe analysis. Digestible energy (DE), ME, and NFE levels were also calculated. Means (dry-matter basis) and coefficients of variation for each nutrient among all plants during 1997 to 1999 were DM (88.9%, 1.7%), CP (30.2%, 6.4%), crude fat (10.9%, 7.8%), crude fiber (8.8%, 8.7%), ash (5.8%, 14.7%), NFE (45.5%, 6.1%), ADF (16.2%, 28.4%), NDF (42.1%, 14.3%), calculated DE (3,990 kcal/kg, 3.24%), calculated ME (3,749 kcal/kg, 3.28%), Arg (1.20%, 9.1%), His (0.76%, 7.8%), Ile (1.12%, 8.7%), Leu (3.55%, 6.4%), Lys (0.85%, 17.3%), Met (0.55%, 13.6%), Phe (1.47%, 6.6%), Thr (1.13%, 6.4%), Trp (0.25%, 6.7%), Val (1.50%, 7.2%), Ca (0.06%, 57.2%), and P (0.89%, 11.7%), respectively. Among the amino acids analyzed, Lys was the most variable (CV = 17.3%), followed by Met (CV = 13.6%). Nutrient levels of MNSD DDGS were higher in crude fat, NDF, DE, ME, P, Lys, Met, and Thr and lower for DM, ADF, and Ca than NRC (1998) values. Nutrient values differed between years for ash, DE, Mn, Zn, Cys (P < 0.10), Fat, TDN, ME, Met, Ile (P < 0.05), Ca, P, K, Mg, and Cu (P < 0.01). These results suggest that gross energy; P; and total Lys, Met, and Thr levels are higher in DDGS from MNSD ethanol plants compared to published values and chemical analysis values of a DDGS sample obtained from an older Midwestern plant.

Some sources of corn dried distillers grains with solubles (DDGS) contain relatively high amounts of oxidized lipids produced from PUFA peroxidation during the production process. These oxidized lipids may impair metabolic oxidation status of pigs. The objective of this study was to understand the effects of feeding corn-soybean meal diets (CON) or diets containing 30% highly oxidized DDGS with 1 of 3 levels of supplemental vitamin E (dl-α-tocopheryl acetate), none, the 1998 NRC level (11 IU/kg), and 10x the 1998 NRC level (110 IU/kg), on oxidative status of nursery pigs. The DDGS source used in this study contained the greatest thiobarbituric acid reactive substances (TBARS) value, peroxide value, and total S content (5.2 ng/mg oil, 84.1 mEq/kg oil, and 0.95%, respectively) relative to 30 other DDGS sources sampled (mean values = 1.8 ng/mg oil, 11.5 mEq/kg oil, and 0.50%, respectively). Barrows (n = 54) were housed in pens and fed the experimental diets for 8 wk after weaning and transferred to individual metabolism cages for collection of feces, urine, blood, and liver samples. Total S content was greater in DDGS diets than in CON (0.39 vs. 0.19%). Dietary inclusion of 30% DDGS improved apparent total tract digestibility of S (86.8 vs. 84.6%; P < 0.001) and S retained (2.94 vs. 2.07 g/d; P < 0.01) compared with CON. Although pigs were fed highly oxidized DDGS in this study, serum TBARS were similar between DDGS and CON treatments. There was an interaction between DDGS and dietary vitamin E level for serum concentrations of α-tocopherol. Serum α-tocopherol concentrations were greater (P < 0.001) in pigs fed DDGS diets than those fed CON when dl-α-tocopheryl acetate was not provided or provided at the NRC level but were similar when dl-α-tocopheryl acetate was supplemented at the 10x NRC level. Pigs fed DDGS diets had greater serum concentrations of S-containing AA, particularly Met (P < 0.001) and taurine (P = 0.002), compared with those fed CON. Liver glutathione concentration was greater in pigs fed DDGS diets than CON (56.3 vs. 41.8 nmol/g). Dietary inclusion of DDGS (P < 0.001) and vitamin E (P = 0.03) increased enzyme activity of glutathione peroxidase. The elevated concentrations of S-containing antioxidants (Met, taurine, and glutathione) in vivo may protect pigs against oxidative stress when feeding highly oxidized DDGS. Therefore, the increased S content in DDGS may be beneficial, and increasing concentrations of vitamin E in diets may not be necessary to protect pigs against metabolic oxidative stress when feeding high S and highly peroxidized DDGS.

Lipid peroxidation in feed can negatively affect animal health, growth performance, and meat quality. The objective of this study was to determine the lipid peroxidation level in corn dried distillers grains with solubles (DDGS) samples from 31 U.S. ethanol plants and compare results with a corn sample obtained from a corn processing plant. Lipids from each sample were extracted with hexane and analyzed for peroxide value (PV) and thiobarbituric acid reactive substances (TBARS). Peroxide values of DDGS samples ranged from 4.2 to 84.1 milliequivalents (meq)/kg oil (CV = 97.5%). The greatest PV among DDGS samples was 27 times greater than that of the corn sample (3.1 meq/kg oil). The TBARS values for DDGS samples ranged from 1.0 to 5.2 ng malondialdehyde (MDA) equivalents/mg oil (CV = 43.6%). The DDGS sample with the greatest TBARS value was 25 times greater than that of the corn sample (0.2 ng MDA equivalents/mg oil). Color of DDGS samples was measured by Minolta L*, a*, and b*, corresponding to the degree of lightness, redness, and yellowness, respectively. Correlations between PV, TBARS, and color were determined. Values of PV and TBARS were correlated positively (r = 0.81; P < 0.001). Both TBARS and PV were correlated negatively with L* (r = -0.73; P < 0.001, and r = -0.63; P < 0.001, respectively) and b* (r = -0.67; P < 0.001, and r = -0.57; P < 0.001, respectively), which suggests that darker and less yellow-colored DDGS samples were more likely to have a greater lipid peroxidation level, as measured by TBARS and PV, compared with lighter or more yellow-colored DDGS samples. However, a* was not correlated with either PV (P = 0.97) or TBARS (P = 0.66). These results indicate that color can be a preliminary indicator of lipid peroxidation level in DDGS, but a more reliable assessment of peroxidation level is achieved by measuring PV and TBARS.

Feeding high-fiber diets decreases cost, but also caloric and nutritional efficiency by modifying intestinal morphology and function. We analyzed the changes in intestinal cell composition, nutrient transporters and receptors, and cell differentiation induced by fibers from different sources. Forty-six finishing pigs (BW 84 ± 7 kg) were fed 1 of 4 diets: corn-soybean (Control; = 12), 23% wheat straw (WS; = 11), 55% corn distillers dried grains with solubles (DDGS; = 11), and 30% soybean hulls (SBH; = 12). Pigs were fed 2 meals daily to an amount equivalent to 2.5% of initial BW for 14 d in metabolism cages. Ilea were collected for histological and gene expression analysis after euthanasia. Data were analyzed using the Kruskal-Wallis test followed by Dunn's multiple comparisons and differences considered significant when < 0.05. The enterocyte marker was increased ( < 0.03) by feeding SBH compared with Control and WS diets. Goblet cells presence was greater ( < 0.01) in pigs fed WS and DDGS compared with Control, and in pigs fed WS compared with SBH ( = 0.02). expression was greater ( < 0.05) in pigs fed DDGS and SBH compared with Control diet. No changes were observed for endocrine and Paneth cells markers, villus and crypt length, or proliferation index. Compared with the Control, gene expression of receptors for oligopeptides, calcium, glucose, fructose, , and and was increased ( < 0.05) by feeding WS and DDGS diets. Feeding SBH diet repressed ( < 0.005) the compared with WS and DDGS diets, while DDGS repressed ( = 0.02) its expression compared with Control. Pigs fed DDGS had reduced ( < 0.001) , and those fed SBH showed increased ( < 0.05) expression compared with WS and DDGS pigs. Feeding WS and DDGS diets induced ( < 0.01) the expression of stem cell marker r-spondin receptor (, while was reduced ( < 0.02) by feeding DDGS compared with Control. The expression of was induced ( < 0.05) by all fibers compared with Control. Transcription factors and were suppressed ( < 0.001) by WS and DDGS compared with Control. In conclusion, feeding diets containing WS and DDGS modulated intestinal differentiation by promoting goblet cells and altered expression of nutrient receptors and transporters in growing pigs, while feeding SBH had less effect.

The ability of enzymes, direct-fed microbials, or yeast to enhance nutrient utilization or growth performance in nursery or finishing pigs fed diets containing increased levels of fiber from corn distillers dried grains with solubles is largely unknown. A total of 192 nursery (11.9 kg initial BW) and 96 finishing (98.4 kg initial BW) pigs were allotted to individual pens and fed their respective diets for 5 wk. Diets containing corn, soybean meal, and 30% corn distillers dried grains with solubles were adequate in all nutrients and were offered ad libitum in meal form. Additives were included at the recommended rates and were assumed to contain the active ingredients and activity level listed on the product label. In the starter experiment, Allzyme and Releezyme decreased GE, N, C, P, ADF, and NDF digestibility (P < 0.05), whereas Econase decreased S, P, and NDF digestibility (P < 0.05). In the finisher experiment, Allzyme increased P digestibility (P < 0.05), BactoCel decreased N digestibility (P = 0.05), BioPlus2B decreased ether extract digestibility (P < 0.05), Hemicel decreased ADF digestibility (P < 0.05), Porzyme decreased GE, N, C, S, P, ADF, and NDF digestibility (P < 0.05), Releezyme decreased GE, N, C, S, P, ADF, and ether extract digestibility (P < 0.05), and XPC yeast decreased GE and C digestibility (P = 0.05). No effect on nursery-or finishing-pig growth performance because of any feed additive was noted (P > 0.10). In conclusion, even though some of the feed additive products evaluated had small effects on nutrient digestibility, none of the products affected starter-and finishing-pig growth performance when fed nutritionally adequate corn-soy diets containing 30% corn distillers dried grains with solubles.