Single-stranded fragments of adenovirus type 2 DNA were isolated from infected KB cells under conditions which retarded reassociation of complementary sequences but did not denature native viral DNA. Of the total intracellular, virus-specific DNA labeled during a 1-h pulse with tritiated thymidine beginning 15 h after infection, about 20% was single stranded when fractionated on hydroxylapatite. This DNA shifted predominantly to the double-stranded fraction on hydroxylapatite during an extended chase incubation, suggesting that it may represent single-stranded DNA in replicating intermediates. Furthermore, the single-stranded DNA annealed nearly equally to both strands of the adenovirus genome. These findings indicate that at least portions of both complementary strands of adenovirus type 2 DNA are exposed as single strands during the period of viral DNA synthesis.
Spleen cells from mice infected with scrapie virus were separated into subpopulations on the basis of buoyant density in discontinuous gradients of isotonic albumin or differential adherence of cells to plastic. At three different intervals after infection, a population of “less dense” cells was found in albumin gradients that had 40-to 60-fold higher specific infectivity (cells per median lethal dose) than the total cell suspension before gradient sedimentation. The class of cells associated with high relative specific infectivity has a density characteristic of lymphoblasts, myeloblasts, and macrophages. Separation of “macrophage rich” cells on the basis of adherence to plastic did not result in significant enrichment of scrapie virus-infected cells.
Progressive pneumonia virus, the causative agent of a slow, pulmonary disease of Montana sheep, was shown to be antigenically related to two other slow viruses of sheep, visna and maedi. Electron microscopic examination of infected cells revealed that the virus matures by a budding process and that the budding particles as well as the mature, extracellular virions bear striking resemblances to the oncogenic ribonucleic acid (RNA) viruses. Recent findings of an RNA-dependent deoxyribonucleic acid polymerase associated with the virions of this group of slow viruses lend further support to the notion that they may tentatively be classified with the oncogenic RNA tumor viruses.
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