Owing to an increasing interest in nutrition and the introduction of certain statutory requirements, there is a need for methods for the determination of a broad spectrum of micronutrients. One of these is vitamin B,, (cyanocobalamin), which is widely distributed in foods of animal origin but is absent from those derived from plants. There is current interest in the use of vegetable proteins as a partial replacement for meat in meat products. Various authorities, such as the Food Standards Committee in the UK and the Food and Drug
In this paper, a novel spectroelectrochemical method involving transmission spectroscopy parallel to an electrode surface, in combination with chronoamperometry and linear-sweep voltammetry, is described and assessed.
Experimental ApparatusAn ultravioletvisible spectrometer was constructed from the appropriate components. The cell and electrode assembly consisted of a 2-cm silica cell containing two platinum-plate electrodes mounted horizontally. The cell was fitted with an inlet for oxygen-free nitrogen, a salt bridge (for connection with a saturated calomel reference electrode) and an outlet to remove solutions by suction. A potentiostatic waveform generator was used as a potential source.
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