A low-cost and green-synthesized zinc oxide nanostructured particles are extensively studied owing
to their remarkable and ample characteristics with less toxicity and eco-friendly approach. The present
work comprehends the green synthesis of ZnO nanostructured particles using bougainvillea leaf
extract-arbitrated microwave-assisted synthesis and their use in field effect transistor for nitrogen
dioxide sensing at room temperature. The as-synthesized nanoparticles were characterized using
analytical techniques; XRD determined the pure crystallite structure with no impurities, SEM confirmed
the spherical shape of nanoparticles with ~20 nm (average particle size) and the atomic weight
percentage were analyzed using EDAX, notable photophysical properties were revealed from absorption
and emission spectra performed using UV-visible spectroscopy. Poly(3-hexylthiophene) and ZnO
nanoparticles were employed in the field effect transistor (p-type) for NO2 sensing at room temperature
with the mobility (field-effect) of ~10-4 cm2 V-1 s-1. The sensitivity of the fabricated OFET device was
extracted from the transistor characteristics (at Vgs = -30 V and Vds = -40 V) found to be ~4.8 × 10-3
nA/ppm. The device exhibited engrossing characteristics such as excellent recoverability (> 95%),
with ultrafast response time (< 30 s) and greater sensitivity with high stability as can be assessed from
the electrical characteristics.
This chapter contributes to the study of uncertainty of signal dimensions within a microscopic image of blood sample. Appropriate colocalization indicator classifies the leukocytes in the region of interest having ragged boundaries. Signal transduction has been interpreted using correlation function determined fluorescence intensity in proposed work using just another colocalization plugin (JaCoP). Dependence between the channels in the colocalization region is being analysed in a linear fashion using Pearson correlation coefficient. Manders split, which gives intensity, is represented in a channel by co-localizing pixels. Overlap coefficients are also being analysed to analyse coefficient of each channel. Li's intensity correlation coefficient is being used in specific cases to interpret the impact of staining.
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