Ihe 'H-NMR spin-spin relaxation time C/',) i" Triticale seeds swelling in external osmotica, polyethylene j-lycol 8000 or miiiinitol can identity both bound and free water. At the same water content, the free water spinspin relaxation time increases lor seeds inihibed with the mannilol solution, demonstrating inadeqnale water potential adjustment. Ihe exchange rate ol Iree/bound water molecules is apparently influenced by the driving forte lor water flow. Ihe reciprocal liletime of Tree water molecules, as a measure of water How through the main cell harrier, was obtained. From a model of the seed as a resistance-capacitor network for water How, ii method was derived lor calculating the reflection coel'ficit'iit a as a lifetime ratio of the free water molecules in seeds imbibed with two (IKTerent osmotica (one penetrating across the main cell barrier and one tiot penetratinj;) a( the same water potential. The 'H-NMR method and the classical method based on volume rate changes yielded reCleclion coefficients for mannitol for the tell wall-pliisnialemnui barrier ol' 0-78 ± 0-08 and 0 68 ± 0()6, respectively. Kev-\vot(ls: lritic(ttc sccil; bound water; cell wall-plastnalcniiiia barrier; lice water; lifetime o\' water tnoleeule; pulse 'H-NMR; refleetioii eoefficieiit; spin-spin relaxatkm time; steady-state flow; water potetitiul. Chemistry (fl, 1328Chemistry (fl, -1333
Abstract— At 293 K the long‐wavelength absorption and emission band of 1.4 μM allophycocyanin is decreased by estriol (Δ1‐3‐5(10)‐estratriene‐3,16α, 17β‐trio!) in the range 0.8‐6.6 μM in the presence of 11% alcohol (vol/vol). The binding of estriol is shown to be of high affinity, 1:1 with allophycocyanin. The free energy of this binding process (ΔG°) is ‐33.6 kJ mol' and single binding site dissociation constant (KD) 1.0 ×10–6M. Estriol at 21 μM effectively quenches the fluorescence of 1.4 M large molecular weight phytochrome in its red absorbing form at 77 K while having little or no effect on the phototransformation difference spectrum at 293 K.
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