Testicular development and spermatogenesis are tightly regulated by the number of genes and noncoding genes, and mRNAs and lncRNAs play vital roles in regulating posttranscriptional gene expression. However, mRNAs and lncRNAs have not been systematically identified in the testes of donkeys. In this study, mRNA and lncRNA expression profiles in the testes of DeZhou donkeys between 2 months and 2 years of age were comprehensively analysed by RNA sequencing. We identified 56,605 lncRNAs and 61,857 mRNAs by gene expression analysis, and 21,845 lncRNAs (p < .05) and 14,109 mRNAs (p < .05) were differentially expressed in the immature (2‐month‐old, n = 3, noADGW) and mature (2‐year‐old, n = 3, ADGW) stages. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the predicted target genes were enriched in the adherens junction, cell cycle, propanoate metabolism and cell adhesion molecule pathways. This study identified and analysed a comprehensive catalogue of lncRNAs and mRNAs in donkey testes, which provides a useful resource for further investigation of biological function in donkey lncRNAs.
Semen cryopreservation has played an important role in popularizing advanced Assisted Reproductive Technologies (ARTs) such as artificial insemination of donkeys. However, cryopreservation is also an important factor affecting the sperm fertilization rate, and the fertility rate of donkey semen after cryopreservation is significantly decreased (Rota et al., 2012). Freezing and thawing can lead to damage to several sperm (Ugur et al., 2019), pre-mature sperm capacitation (Cormier et al., 1997;Longobardi et al., 2017) and the loss of fertility-related molecules (Lone et al., 2017).In the process of freezing-thawing, metabolites can affect the viability of sperm and oxidative stress. For example, phosphatidylcholine in the plasma membrane of human sperm is directly related
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.