The epidermal growth factor receptor (EGFR) family belongs to type I receptor tyrosine kinases. Overexpression or mutation of EGFR/ErbB1 gene has been detected in a large number of human solid tumours. According to some previous report, this gene is not expressed in hematological malignancies. However, two recent clinical case reports showed that erlotinib caused complete remission of acute myeloid leukaemia (AML)-M1 in patients who had both AML-M1 and non-small-cell lung cancer. These results are supported by preclinical studies in which EGFR tyrosine kinase inhibitors have anti-proliferative effects on AML. These findings prompted us to determine whether EGFR is expressed in human AML, through a large-scale screening of both leukaemic cell lines and clinical samples. Our results show that EGFR is expressed by about 33% of human AML (containing M1 to M7 subtypes) and by some human leukaemia cell lines (K562, MEG-01, CEM and SKO-007). Its expression is not limited to certain AML types but has been detected in many leukaemic cells. In addition, EGFR expression was intimately associated with the poor clinical outcomes. Finally, we find that only EGFR-positive leukaemic cells respond to antibody-dependent cellular cytotoxicity of cetuximab, the monoclonal antibodies against EGFR.

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We report a high-intensity laser facility named Xingguang-III that generates femtosecond, picosecond, and nanosecond beams with three wavelengths, i.e. 800 nm, 1053 nm, and 527 nm, respectively. To the best of our knowledge, the laser facility is the first one which produces three beams with different pulse widths and wavelengths. An optical synchronization technique, combining super continuum generation and femtosecond optical parametric amplification, was developed to ensure three beams are from the same source to achieve precise synchronization. The femtosecond beam is a double chirped-pulseamplification Ti:sapphire laser which applies cross-polarized wave generation to improve the temporal contrast. The picosecond/nanosecond beams utilize the optical parametric amplification + Nd:glass mixed amplification scheme. The output energy and pulse width of the three beams are 20.1 J/26.8 fs, 370.2 J/0.48 ps (shortest), and 575.4 J/1.0 ns, respectively. The smallest synchronization time (peak-to-valley) and the shot-to-shot timing jitter (peak-topeak) of less than 1.32 ps have been achieved for the femtosecond and picosecond beams.